8YKD

Cryo-EM structure of ADGRG2-Gs complex with NTF nanobody

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(s) subunit alpha	polymer	361	41879.5	1	UniProt (P63091) Pfam (PF00503)	Spodoptera
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	358	39418.1	1	UniProt (P62871) Pfam (PF00400)	Spodoptera	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein subunit gamma	polymer	108	12104.9	1	UniProt (A0A7J7XNR4) Pfam (PF00631)	Spodoptera
4	D (N)	Nanobody-35	polymer	128	13885.4	1		Spodoptera
5	E (R)	Adhesion G-protein coupled receptor G2	polymer	903	98934.8	1	UniProt (Q8CJ12) Pfam (PF01825) Pfam (PF00002)	Spodoptera	G-protein coupled receptor 64,Mouse epididymis-specific protein 6,Me6
6	F (S)	ScFv-16	polymer	250	26640.6	1		Spodoptera
7	G (R)	3-BETA-HYDROXY-5-ANDROSTEN-17-ONE	non-polymer		288.4	1	Chemie (AND)

Sequence modifications

A: 18 - 213 (PDB: 8YKD)
A: 214 - 394 (UniProt: P63091)

PDB	External Database	Details
Ala 236	Gly 226	conflict
Asp 259	Ala 249	conflict
Asp 262	Ser 252	conflict
-	Asn 254	deletion
-	Met 255	deletion
-	Val 256	deletion
-	Ile 257	deletion
-	Arg 258	deletion
-	Glu 259	deletion
-	Asp 260	deletion
-	Asn 261	deletion
-	Gln 262	deletion
-	Thr 263	deletion
Asp 272	Leu 272	conflict
Ser 366	Ala 366	conflict
Ala 372	Ile 372	conflict
Ile 375	Val 375	conflict

B: 2 - 340 (UniProt: P62871)

PDB	External Database	Details
Met -17	-	initiating methionine
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

G: -9 - 71 (UniProt: A0A7J7XNR4)

PDB	External Database	Details
Met -36	-	initiating methionine
Trp -35	-	expression tag
Arg -34	-	expression tag
Glu -33	-	expression tag
Leu -32	-	expression tag
Pro -31	-	expression tag
Leu -30	-	expression tag
Gly -29	-	expression tag
Leu -28	-	expression tag
Gly -27	-	expression tag
Glu -26	-	expression tag
Leu -25	-	expression tag
His -24	-	expression tag
Lys -23	-	expression tag
Asp -22	-	expression tag
His -21	-	expression tag
Gln -20	-	expression tag
Ala -19	-	expression tag
Ser -18	-	expression tag
Arg -17	-	expression tag
Lys -16	-	expression tag
Leu -15	-	expression tag
Glu -14	-	expression tag
Pro -13	-	expression tag
Glu -12	-	expression tag
Leu -11	-	expression tag
Trp -10	-	expression tag

R: 38 - 891 (UniProt: Q8CJ12)

PDB	External Database	Details
Met 14	-	initiating methionine
Lys 15	-	expression tag
Thr 16	-	expression tag
Ile 17	-	expression tag
Ile 18	-	expression tag
Ala 19	-	expression tag
Leu 20	-	expression tag
Ser 21	-	expression tag
Tyr 22	-	expression tag
Ile 23	-	expression tag
Phe 24	-	expression tag
Cys 25	-	expression tag
Leu 26	-	expression tag
Val 27	-	expression tag
Phe 28	-	expression tag
Ala 29	-	expression tag
Asp 30	-	expression tag
Tyr 31	-	expression tag
Lys 32	-	expression tag
Asp 33	-	expression tag
Asp 34	-	expression tag
Asp 35	-	expression tag
Asp 36	-	expression tag
Lys 37	-	expression tag
Ala 597	His 597	engineered mutation
Ala 599	Thr 599	engineered mutation
Phe 892	-	expression tag
Trp 893	-	expression tag
Phe 894	-	expression tag
Pro 895	-	expression tag
Glu 896	-	expression tag
Lys 897	-	expression tag
Gly 898	-	expression tag
Ala 899	-	expression tag
Ile 900	-	expression tag
Leu 901	-	expression tag
Thr 902	-	expression tag
Asp 903	-	expression tag
Thr 904	-	expression tag
Ser 905	-	expression tag
Val 906	-	expression tag
Lys 907	-	expression tag
Arg 908	-	expression tag
Asn 909	-	expression tag
Asp 910	-	expression tag
Leu 911	-	expression tag
Ser 912	-	expression tag
Ile 913	-	expression tag
Ile 914	-	expression tag
Ser 915	-	expression tag
Gly 916	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	232863.3
Non-Polymers*	Number of molecules	1
	Total formula weight	288.4
All*	Total formula weight	233151.8

*Water molecules are not included.