8YK3

Blood group B alpha-1,3-galactosidase AgaBb from Bifidobacterium bifidum, construct T7-tag_24-673

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Alpha-galactosidase	polymer	673	72590.4	2	UniProt (L8B3G2) Pfam (PF13229)	Bifidobacterium bifidum JCM 1254
2	C, H (A, B)	alpha-D-galactopyranose	non-polymer		180.2	2	Chemie (GLA)
3	D, E, F (A)	1,2-ETHANEDIOL	non-polymer		62.1	3	Chemie (EDO)
4	G (B)	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)

Sequence modifications

A, B: 24 - 673 (UniProt: L8B3G2)

PDB	External Database	Details
Met 9	-	initiating methionine
Ala 10	-	expression tag
Ser 11	-	expression tag
Met 12	-	expression tag
Thr 13	-	expression tag
Gly 14	-	expression tag
Gly 15	-	expression tag
Gln 16	-	expression tag
Gln 17	-	expression tag
Met 18	-	expression tag
Gly 19	-	expression tag
Arg 20	-	expression tag
Asp 21	-	expression tag
Pro 22	-	expression tag
Asn 23	-	expression tag
Leu 674	-	expression tag
Glu 675	-	expression tag
His 676	-	expression tag
His 677	-	expression tag
His 678	-	expression tag
His 679	-	expression tag
His 680	-	expression tag
His 681	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	145180.7
Non-Polymers*	Number of molecules	6
	Total formula weight	741.8
All*	Total formula weight	145922.5

*Water molecules are not included.