8YJO

Structure of E. coli glycyl radical enzyme PflD with bound malonate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Probable dehydratase PflD	polymer	781	87748.3	2	UniProt (P32674) Pfam (PF02901) Pfam (PF01228)	Escherichia coli (strain K12)
2	C, D (A, B)	MALONATE ION	non-polymer		102.0	2	Chemie (MLI)
3	E, F (A, B)	water	water		18.0	1052	Chemie (HOH)

Sequence modifications

A, B: 1 - 765 (UniProt: P32674)

PDB	External Database	Details
Met -15	-	initiating methionine
Gly -14	-	expression tag
Ser -13	-	expression tag
Ser -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
Ser -5	-	expression tag
Gln -4	-	expression tag
Ser -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	175496.5
Non-Polymers*	Number of molecules	2
	Total formula weight	204.1
All*	Total formula weight	175700.6

*Water molecules are not included.