8YIC

SAR247799-bound S1PR1-Gi protein complex

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	364	41601.3	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	343	37574.0	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (R)	Endolysin,Sphingosine 1-phosphate receptor 1	polymer	515	58457.5	1	UniProt (P00720) UniProt (P21453) Pfam (PF00959) Pfam (PF00001)	Enterobacteria phage T4 (Bacteriophage T4)	Lysis protein,Lysozyme,Muramidase,S1P receptor 1,S1P1,Endothelial differentiation G-protein coupled receptor 1,Sphingosine 1-phosphate receptor Edg-1,S1P receptor Edg-1
5	E (R)	SAR247799	non-polymer		425.8	1	Chemie (A1LYQ)

Sequence modifications

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Glu -9	-	expression tag
Gln -8	-	expression tag
Lys -7	-	expression tag
Leu -6	-	expression tag
Ile -5	-	expression tag
Ser -4	-	expression tag
Glu -3	-	expression tag
Glu -2	-	expression tag
Asp -1	-	expression tag
Leu 0	-	expression tag

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

R: -159 - 0 (UniProt: P00720)

PDB	External Database	Details
Asp -167	-	expression tag
Tyr -166	-	expression tag
Lys -165	-	expression tag
Asp -164	-	expression tag
Asp -163	-	expression tag
Asp -162	-	expression tag
Asp -161	-	expression tag
Ala -160	-	expression tag
Gly -149	Arg 12	engineered mutation
Asn -141	Asp 20	engineered mutation
Thr -107	Cys 54	engineered mutation
Ala -64	Cys 97	engineered mutation
Arg -24	Ile 137	engineered mutation

R: 1 - 337 (UniProt: P21453)

PDB	External Database	Details
His 338	-	expression tag
His 339	-	expression tag
His 340	-	expression tag
His 341	-	expression tag
His 342	-	expression tag
His 343	-	expression tag
His 344	-	expression tag
His 345	-	expression tag
His 346	-	expression tag
His 347	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	145493.9
Non-Polymers*	Number of molecules	1
	Total formula weight	425.8
All*	Total formula weight	145919.7

*Water molecules are not included.