8YH3

A3R-Gi complex bound to m6A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	375	40586.3	1	UniProt (P62871) Pfam (PF00400)	Rattus rattus (black rat)	Transducin beta chain 1
2	B (R)	Hemagglutinin,Adenosine receptor A3,GFP chimera	polymer	794	88315.9	1	UniProt (P03435) UniProt (W5QED6) UniProt (A0A5P9VSM6) Pfam (PF00001) Pfam (PF01353)	Influenza A virus (A/Victoria/3/1975(H3N2))
3	C, E (A, G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2,Guanine nucleotide-binding protein G(i) subunit alpha-1 chimera	polymer	433	48846.7	2	UniProt (P59768) UniProt (P63097) Pfam (PF00631) Pfam (PF00503)	Homo sapiens (human)	G gamma-I,Adenylate cyclase-inhibiting G alpha protein
4	D (S)	scfv16	polymer	260	27720.8	1		Mus musculus
5	F (R)	N-methyladenosine	non-polymer		281.3	1	Chemie (6MD)

Sequence modifications

B: 2 - 340 (UniProt: P62871)

PDB	External Database	Details
Met -4	-	initiating methionine
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag
Gly 341	-	expression tag
Ala 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Ser 347	-	expression tag
Gly 348	-	expression tag
Gly 349	-	expression tag
Asn 350	-	expression tag
Ser 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Ser 354	-	expression tag
Gly 355	-	expression tag
Gly 356	-	expression tag
Ser 357	-	expression tag
Ser 358	-	expression tag
Gly 359	-	expression tag
Val 360	-	expression tag
Ser 361	-	expression tag
Gly 362	-	expression tag
Trp 363	-	expression tag
Arg 364	-	expression tag
Leu 365	-	expression tag
Phe 366	-	expression tag
Lys 367	-	expression tag
Lys 368	-	expression tag
Ile 369	-	expression tag
Ser 370	-	expression tag

R: -24 - -9 (UniProt: P03435)

PDB	External Database	Details
Asp -8	-	linker
Tyr -7	-	linker
Lys -6	-	linker
Asp -5	-	linker
Asp -4	-	linker
Asp -3	-	linker
Asp -2	-	linker
Ala -1	-	linker
Met 0	-	linker
Gly 1	-	linker

R: 2 - 317 (UniProt: W5QED6)

PDB	External Database	Details
Gly 318	-	linker
Ser 319	-	linker
Gly 320	-	linker
Gly 321	-	linker
Gly 322	-	linker
Gly 323	-	linker
Ser 324	-	linker
Gly 325	-	linker
Gly 326	-	linker
Ser 327	-	linker
Ser 328	-	linker
Ser 329	-	linker
Gly 330	-	linker
Gly 331	-	linker
Gly 490	-	linker
Gly 491	-	linker
Ser 492	-	linker
Gly 493	-	linker
Gly 494	-	linker
Gly 495	-	linker
Gly 496	-	linker
Ser 497	-	linker
Gly 498	-	linker
Gly 499	-	linker
Ser 500	-	linker
Ser 501	-	linker
Ser 502	-	linker
Gly 503	-	linker
Gly 504	-	linker
Leu 505	-	linker
Glu 506	-	linker
Val 507	-	linker
Leu 508	-	linker
Phe 509	-	linker
Gln 510	-	linker
Gly 511	-	linker
Pro 512	-	linker
Gly 513	-	linker
Ser 514	-	linker
Ala 515	-	linker
Ala 516	-	linker
Ala 517	-	linker
Ala 518	-	linker

R: 332 - 489 (PDB: 8YH3)
R: 519 - 756 (UniProt: A0A5P9VSM6)

PDB	External Database	Details
Ser 757	-	expression tag
Gly 758	-	expression tag
Leu 759	-	expression tag
Arg 760	-	expression tag
Ser 761	-	expression tag
His 762	-	expression tag
His 763	-	expression tag
His 764	-	expression tag
His 765	-	expression tag
His 766	-	expression tag
His 767	-	expression tag
His 768	-	expression tag
His 769	-	expression tag

A, G: -78 - -8 (UniProt: P59768)

PDB	External Database	Details
Gly -7	-	linker
Ser -6	-	linker
Ala -5	-	linker
Gly -4	-	linker
Ser -3	-	linker
Ala -2	-	linker
Gly -1	-	linker
Ser 0	-	linker
Ala 1	-	linker
Met 2	-	linker

A, G: 3 - 354 (UniProt: P63097)

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	254316.4
Non-Polymers*	Number of molecules	1
	Total formula weight	281.3
All*	Total formula weight	254597.7

*Water molecules are not included.