8YF5
Cryo EM structure of Komagataella phaffii Rat1-Rai1-Rtt103 complex
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, C | 5'-3' exoribonuclease | polymer | 1006 | 115314.5 | 2 | UniProt (F2QV79) In PDB | Komagataella phaffii (Yeast Pichia pastoris) | |
2 | B, D | Decapping nuclease | polymer | 384 | 44596.0 | 2 | UniProt (F2QLF5) In PDB | Komagataella phaffii (Yeast Pichia pastoris) | |
3 | E | Exonuclease Rat1p and Rai1p interacting protein | polymer | 381 | 42272.9 | 1 | UniProt (F2QNA8) In PDB | Komagataella phaffii (Yeast Pichia pastoris) |
Sequence modifications
A, C: 1 - 994 (UniProt: F2QV79)
B, D: 1 - 381 (UniProt: F2QLF5)
E: 1 - 375 (UniProt: F2QNA8)
PDB | External Database | Details |
---|---|---|
Ala 233 | Asp 233 | engineered mutation |
Ala 235 | Asp 235 | engineered mutation |
Ser 995 | - | expression tag |
Gly 996 | - | expression tag |
Pro 997 | - | expression tag |
Ser 998 | - | expression tag |
Ser 999 | - | expression tag |
Gly 1000 | - | expression tag |
Leu 1001 | - | expression tag |
Glu 1002 | - | expression tag |
Val 1003 | - | expression tag |
Leu 1004 | - | expression tag |
Phe 1005 | - | expression tag |
Gln 1006 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Gly -2 | - | expression tag |
Pro -1 | - | expression tag |
Gly 0 | - | expression tag |
Ala 213 | Glu 213 | engineered mutation |
Ala 215 | Asp 215 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Leu 376 | - | expression tag |
Glu 377 | - | expression tag |
Val 378 | - | expression tag |
Leu 379 | - | expression tag |
Phe 380 | - | expression tag |
Gln 381 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 5 |
Total formula weight | 362093.8 | |
All* | Total formula weight | 362093.8 |