8YF5

Cryo EM structure of Komagataella phaffii Rat1-Rai1-Rtt103 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	5'-3' exoribonuclease	polymer	1006	115314.5	2	UniProt (F2QV79) Pfam (PF03159) Pfam (PF17846) UniProt (by SIFTS) (C4R5R1)	Komagataella phaffii (Yeast Pichia pastoris)
2	B, D (B, D)	Decapping nuclease	polymer	384	44596.0	2	UniProt (F2QLF5) Pfam (PF08652) UniProt (by SIFTS) (C4QXY9)	Komagataella phaffii (Yeast Pichia pastoris)
3	E (E)	Exonuclease Rat1p and Rai1p interacting protein	polymer	381	42272.9	1	UniProt (F2QNA8) Pfam (PF04818) UniProt (by SIFTS) (C4QXL6)	Komagataella phaffii (Yeast Pichia pastoris)

Sequence modifications

A, C: 1 - 994 (UniProt: F2QV79)

PDB	External Database	Details
Ala 233	Asp 233	engineered mutation
Ala 235	Asp 235	engineered mutation
Ser 995	-	expression tag
Gly 996	-	expression tag
Pro 997	-	expression tag
Ser 998	-	expression tag
Ser 999	-	expression tag
Gly 1000	-	expression tag
Leu 1001	-	expression tag
Glu 1002	-	expression tag
Val 1003	-	expression tag
Leu 1004	-	expression tag
Phe 1005	-	expression tag
Gln 1006	-	expression tag

B, D: 1 - 381 (UniProt: F2QLF5)

PDB	External Database	Details
Gly -2	-	expression tag
Pro -1	-	expression tag
Gly 0	-	expression tag
Ala 213	Glu 213	engineered mutation
Ala 215	Asp 215	engineered mutation

E: 1 - 375 (UniProt: F2QNA8)

PDB	External Database	Details
Leu 376	-	expression tag
Glu 377	-	expression tag
Val 378	-	expression tag
Leu 379	-	expression tag
Phe 380	-	expression tag
Gln 381	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	362093.8
All*	Total formula weight	362093.8

*Water molecules are not included.