8YAS

Structure of the SecA-SecY complex with the substrate HmBRI-7TM

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Protein translocase subunit SecA	polymer	778	88673.9	1	UniProt (P28366) Pfam (PF07517) Pfam (PF01043) Pfam (PF21090) Pfam (PF07516)	Bacillus subtilis subsp. subtilis str. 168
2	B (Y)	Protein translocase subunit SecY	polymer	430	47512.1	1	UniProt (A4IJK8)	Geobacillus thermodenitrificans NG80-2
3	C (E)	Protein translocase subunit SecE	polymer	70	8249.6	1	UniProt (A4IJH4)	Geobacillus thermodenitrificans NG80-2
4	D (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
5	E (A)	BERYLLIUM TRIFLUORIDE ION	non-polymer		66.0	1	Chemie (BEF)
6	F (A)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)

Sequence modifications

Y: 1 - 430 (UniProt: A4IJK8)

PDB	External Database	Details
Cys 60	Gly 60	engineered mutation
Thr 202	Gln 202	engineered mutation
Thr 211	Phe 211	engineered mutation
Asn 213	Arg 213	engineered mutation

E: 1 - 60 (UniProt: A4IJH4)

PDB	External Database	Details
Gly 61	-	expression tag
Gly 62	-	expression tag
His 63	-	expression tag
His 64	-	expression tag
His 65	-	expression tag
His 66	-	expression tag
His 67	-	expression tag
His 68	-	expression tag
His 69	-	expression tag
His 70	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	144435.6
Non-Polymers*	Number of molecules	3
	Total formula weight	517.5
All*	Total formula weight	144953.1

*Water molecules are not included.