8YA3

Structure of the SecA-SecY complex with the substrate FtsQ-LacY(+7C) treated with DTT

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (Y)	Protein translocase subunit SecY	polymer	430	47512.1	1	UniProt (A4IJK8) Pfam (PF00344)	Geobacillus thermodenitrificans NG80-2
2	B (E)	Protein translocase subunit SecE	polymer	70	8249.6	1	UniProt (A4IJH4) Pfam (PF00584)	Geobacillus thermodenitrificans NG80-2
3	C (B)	Cell division protein FtsQ,Lactose permease	polymer	78	8272.5	1	UniProt (P06136) UniProt (P02920)	Escherichia coli K-12	Lactose-proton symport

Sequence modifications

Y: 1 - 430 (UniProt: A4IJK8)

PDB	External Database	Details
Cys 60	Gly 60	engineered mutation
Thr 202	Gln 202	engineered mutation
Thr 211	Phe 211	engineered mutation
Asn 213	Arg 213	engineered mutation

E: 1 - 60 (UniProt: A4IJH4)

PDB	External Database	Details
Gly 61	-	expression tag
Gly 62	-	expression tag
His 63	-	expression tag
His 64	-	expression tag
His 65	-	expression tag
His 66	-	expression tag
His 67	-	expression tag
His 68	-	expression tag
His 69	-	expression tag
His 70	-	expression tag

B: 6 - 25 (UniProt: P06136)

PDB	External Database	Details
Met 1	-	initiating methionine
Ala 2	-	expression tag
Lys 3	-	expression tag
Lys 4	-	expression tag
Thr 5	-	expression tag
Ala 26	-	linker
Gln 27	-	linker
Tyr 28	-	linker
Glu 29	-	linker
Asp 30	-	linker
Gly 31	-	linker
Cys 32	-	linker
Ser 33	-	linker
Gly 34	-	linker

B: 35 - 54 (UniProt: P02920)

PDB	External Database	Details
Ala 53	Cys 333	conflict
Ser 55	-	expression tag
Ile 56	-	expression tag
Ser 57	-	expression tag
Gly 58	-	expression tag
Asp 59	-	expression tag
Gly 60	-	expression tag
Asp 61	-	expression tag
Ser 62	-	expression tag
Pro 63	-	expression tag
His 64	-	expression tag
Ser 65	-	expression tag
Tyr 66	-	expression tag
His 67	-	expression tag
Ser 68	-	expression tag
Gly 69	-	expression tag
Asp 70	-	expression tag
Gly 71	-	expression tag
Asp 72	-	expression tag
Lys 73	-	expression tag
Leu 74	-	expression tag
Pro 75	-	expression tag
Glu 76	-	expression tag
Gly 77	-	expression tag
Val 78	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	64034.2
All*	Total formula weight	64034.2

*Water molecules are not included.