8YA0
Structure of the SecA-SecY complex with the substrate FtsQ-LacY(+7C)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Protein translocase subunit SecA | polymer | 765 | 87182.0 | 1 | UniProt (P28366) Pfam (PF07517) Pfam (PF01043) Pfam (PF21090) Pfam (PF07516) | Bacillus subtilis subsp. subtilis str. 168 | |
| 2 | B (Y) | Protein translocase subunit SecY | polymer | 429 | 47380.9 | 1 | UniProt (A4IJK8) Pfam (PF00344) | Geobacillus thermodenitrificans NG80-2 | |
| 3 | C (E) | Protein translocase subunit SecE | polymer | 58 | 6770.0 | 1 | UniProt (A4IJH4) Pfam (PF00584) | Geobacillus thermodenitrificans NG80-2 | |
| 4 | D (V) | Nanobody | polymer | 116 | 12919.5 | 1 | Lama glama | ||
| 5 | E (B) | Cell division protein FtsQ,Lactose permease | polymer | 72 | 7660.8 | 1 | UniProt (Q7CR81) UniProt (P02920) | Escherichia coli K-12 | Lactose-proton symport |
| 6 | F (C) | Nanobody | polymer | 113 | 12455.8 | 1 | Lama glama | ||
| 7 | G (G) | Green fluorescent protein | polymer | 227 | 25517.6 | 1 | UniProt (P42212) Pfam (PF01353) | Aequorea victoria | |
| 8 | H (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 9 | I (A) | BERYLLIUM TRIFLUORIDE ION | non-polymer | 66.0 | 1 | Chemie (BEF) | |||
| 10 | J (A) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 1 | Chemie (ADP) |
Sequence modifications
Y: 2 - 430 (UniProt: A4IJK8)
B: 2 - 25 (UniProt: Q7CR81)
B: 36 - 52 (UniProt: P02920)
G: 3 - 229 (UniProt: P42212)
| PDB | External Database | Details |
|---|---|---|
| Cys 60 | Gly 60 | engineered mutation |
| Thr 202 | Gln 202 | engineered mutation |
| Thr 211 | Phe 211 | engineered mutation |
| Asn 213 | Arg 213 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ala 2 | Arg 25 | engineered mutation |
| Lys 3 | Leu 26 | engineered mutation |
| Lys 4 | Ala 27 | engineered mutation |
| Thr 5 | Gly 28 | engineered mutation |
| Thr 14 | Cys 37 | engineered mutation |
| Leu 17 | Phe 40 | engineered mutation |
| Ala 26 | - | linker |
| Gln 27 | - | linker |
| Tyr 28 | - | linker |
| Glu 29 | - | linker |
| Asp 30 | - | linker |
| Gly 31 | - | linker |
| Cys 32 | - | linker |
| Ser 33 | - | linker |
| Gly 34 | - | linker |
| Val 35 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Ala 52 | - | linker |
| Phe 52 | - | linker |
| Ser 52 | - | linker |
| Asn 52 | - | linker |
| Ala 52 | - | linker |
| Asp 52 | - | linker |
| Thr 52 | - | linker |
| Ser 52 | - | linker |
| Ile 52 | - | linker |
| Ser 52 | - | linker |
| Gly 52 | - | linker |
| Asp 52 | - | linker |
| Gly 52 | - | linker |
| Asp 61 | - | linker |
| Ser 62 | - | linker |
| Pro 63 | - | linker |
| His 64 | - | linker |
| Ser 65 | - | linker |
| Tyr 66 | - | linker |
| His 67 | - | linker |
| Ser 68 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Gys 66 | Ser 65 | chromophore |
| Gys 66 | Tyr 66 | chromophore |
| Arg 80 | Gln 80 | engineered mutation |
| Ser 99 | Phe 99 | engineered mutation |
| Thr 153 | Met 153 | engineered mutation |
| Ala 163 | Val 163 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 7 |
| Total formula weight | 199886.7 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 517.5 | |
| All* | Total formula weight | 200404.2 |
