8Y9Z

Structure of the SecA-SecY complex with the substrate HmBRI-3TM

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Protein translocase subunit SecA	polymer	778	88673.9	1	UniProt (P28366) Pfam (PF07517) Pfam (PF01043) Pfam (PF21090) Pfam (PF07516)	Bacillus subtilis subsp. subtilis str. 168
2	B (Y)	Protein translocase subunit SecY	polymer	430	47512.1	1	UniProt (A4IJK8)	Geobacillus thermodenitrificans NG80-2
3	C (E)	Protein translocase subunit SecE	polymer	70	8249.6	1	UniProt (A4IJH4)	Geobacillus thermodenitrificans NG80-2
4	D (V)	Nanobody	polymer	117	13006.6	1		Lama glama
5	E (B)	Bacteriorhodopsin-I	polymer	99	11203.1	1	UniProt (Q5UXY6) Pfam (PF01036)	Haloarcula marismortui ATCC 43049	HmBRI
6	F (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
7	G (A)	BERYLLIUM TRIFLUORIDE ION	non-polymer		66.0	1	Chemie (BEF)
8	H (A)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)

Sequence modifications

Y: 1 - 430 (UniProt: A4IJK8)

PDB	External Database	Details
Cys 60	Gly 60	engineered mutation
Thr 202	Gln 202	engineered mutation
Thr 211	Phe 211	engineered mutation
Asn 213	Arg 213	engineered mutation

E: 1 - 60 (UniProt: A4IJH4)

PDB	External Database	Details
Gly 61	-	expression tag
Gly 62	-	expression tag
His 63	-	expression tag
His 64	-	expression tag
His 65	-	expression tag
His 66	-	expression tag
His 67	-	expression tag
His 68	-	expression tag
His 69	-	expression tag
His 70	-	expression tag

B: 1 - 99 (UniProt: Q5UXY6)

PDB	External Database	Details
Cys 75	Pro 75	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	168645.2
Non-Polymers*	Number of molecules	3
	Total formula weight	517.5
All*	Total formula weight	169162.8

*Water molecules are not included.