8Y9Y
Structure of the SecA-SecY complex with the substrate FtsQ-LacY(+1C)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Protein translocase subunit SecA | polymer | 778 | 88673.9 | 1 | UniProt (P28366) Pfam (PF07517) Pfam (PF01043) Pfam (PF21090) Pfam (PF07516) | Bacillus subtilis subsp. subtilis str. 168 | |
| 2 | B (Y) | Protein translocase subunit SecY | polymer | 430 | 47512.1 | 1 | UniProt (A4IJK8) | Geobacillus thermodenitrificans NG80-2 | |
| 3 | C (E) | Protein translocase subunit SecE | polymer | 70 | 8249.6 | 1 | UniProt (A4IJH4) | Geobacillus thermodenitrificans NG80-2 | |
| 4 | D (B) | Substrate FtsQ-LacY(+1C) | polymer | 73 | 7793.0 | 1 | UniProt (P06136) UniProt (P02920) | Escherichia coli K-12 | Lactose-proton symport |
| 5 | E (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 6 | F (A) | BERYLLIUM TRIFLUORIDE ION | non-polymer | 66.0 | 1 | Chemie (BEF) | |||
| 7 | G (A) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 1 | Chemie (ADP) |
Sequence modifications
Y: 1 - 430 (UniProt: A4IJK8)
E: 1 - 60 (UniProt: A4IJH4)
B: 1 - 25 (UniProt: P06136)
B: 35 - 54 (UniProt: P02920)
| PDB | External Database | Details |
|---|---|---|
| Cys 60 | Gly 60 | engineered mutation |
| Thr 202 | Gln 202 | engineered mutation |
| Thr 211 | Phe 211 | engineered mutation |
| Asn 213 | Arg 213 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly 61 | - | expression tag |
| Gly 62 | - | expression tag |
| His 63 | - | expression tag |
| His 64 | - | expression tag |
| His 65 | - | expression tag |
| His 66 | - | expression tag |
| His 67 | - | expression tag |
| His 68 | - | expression tag |
| His 69 | - | expression tag |
| His 70 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met 1 | Thr 23 | engineered mutation |
| Ala 2 | Arg 24 | engineered mutation |
| Lys 3 | Leu 25 | engineered mutation |
| Lys 4 | Ala 26 | engineered mutation |
| Thr 5 | Gly 27 | engineered mutation |
| Cys 26 | - | linker |
| Gln 27 | - | linker |
| Tyr 28 | - | linker |
| Glu 29 | - | linker |
| Asp 30 | - | linker |
| Gly 31 | - | linker |
| Ser 32 | - | linker |
| Ser 33 | - | linker |
| Gly 34 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Ala 53 | Cys 333 | engineered mutation |
| Ser 55 | - | linker |
| Ile 56 | - | linker |
| Ser 57 | - | linker |
| Gly 58 | - | linker |
| Asp 59 | - | linker |
| Gly 60 | - | linker |
| Asp 61 | - | linker |
| Ser 62 | - | linker |
| Pro 63 | - | linker |
| His 64 | - | linker |
| Ser 65 | - | linker |
| Tyr 66 | - | linker |
| His 67 | - | linker |
| Ser 68 | - | linker |
| Gly 69 | - | linker |
| Asp 70 | - | linker |
| Gly 71 | - | linker |
| Asp 72 | - | linker |
| Lys 73 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 152228.5 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 517.5 | |
| All* | Total formula weight | 152746.0 |
