8Y88

Structure of HCoV-HKU1C spike in the functionally anchored-2up conformation with 2TMPRSS2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, C, B)	Spike glycoprotein	polymer	1263	140242.5	3	UniProt (Q0ZME7) Pfam (PF16451) Pfam (PF09408) Pfam (PF19209) Pfam (PF01601)	Human coronavirus HKU1	S glycoprotein,E2,Peplomer protein
2	D, E (T, I)	Transmembrane protease serine 2	polymer	383	42283.6	2	UniProt (O15393) Pfam (PF15494) Pfam (PF00089)	Homo sapiens (human)	Serine protease 10
3	F, H, I (D, F, G)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	3	In PDB GlyTouCan (G42666HT)
4	G (E)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G15407YE)
5	AA, J, K, L, M... (B, A, C)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	18	Chemie (NAG)

Sequence modifications

A, C, B: 14 - 1276 (UniProt: Q0ZME7)

PDB	External Database	Details
Gly 752	Arg 752	engineered mutation
Gly 753	Arg 753	engineered mutation
Ser 754	Lys 754	engineered mutation
Gly 755	Arg 755	engineered mutation
Ser 756	Arg 756	engineered mutation
Pro 902	Leu 902	engineered mutation
Pro 980	Ser 980	engineered mutation
Pro 1023	Asn 1023	engineered mutation
Pro 1067	Asn 1067	engineered mutation
Pro 1068	Leu 1068	engineered mutation

T, I: 110 - 492 (UniProt: O15393)

PDB	External Database	Details
Asp 251	Ser 250	engineered mutation
Asp 252	Ser 251	engineered mutation
-	Arg 252	deletion
Asp 253	Gln 253	engineered mutation
Asp 254	Ser 254	engineered mutation
Lys 255	Arg 255	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	505294.6
Branched	Number of molecules	4
	Total formula weight	1859.7
Non-Polymers*	Number of molecules	18
	Total formula weight	3981.7
All*	Total formula weight	511136.1

*Water molecules are not included.