8Y6U
Cryo-EM structure of E.coli transcription initiation complex with transcription factor GcvA
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (H, J) | Glycine cleavage system transcriptional activator | polymer | 305 | 34447.5 | 2 | UniProt (P0A9F6) Pfam (PF00126) Pfam (PF03466) | Escherichia coli K-12 | Gcv operon activator |
| 2 | C (1) | Non-template promoter DNA | polymer | 92 | 28330.2 | 1 | Escherichia coli | ||
| 3 | D (2) | Template promoter DNA | polymer | 92 | 28584.4 | 1 | Escherichia coli | ||
| 4 | E, F, K (A, B, I) | DNA-directed RNA polymerase subunit alpha | polymer | 329 | 36558.7 | 3 | UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) | Escherichia coli | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 5 | G (C) | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150804.9 | 1 | UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) | Escherichia coli K-12 | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 6 | H (D) | DNA-directed RNA polymerase subunit beta' | polymer | 1407 | 155366.8 | 1 | UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Escherichia coli K-12 | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 7 | I (E) | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (P0A800) Pfam (PF01192) | Escherichia coli K-12 | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 8 | J (F) | RNA polymerase sigma factor RpoD | polymer | 613 | 70352.2 | 1 | UniProt (Q0P6L9) Pfam (PF03979) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) UniProt (by SIFTS) (P00579) | Escherichia coli | Sigma-70 |
Sequence modifications
C: 1 - 1342 (UniProt: P0A8V2)
| PDB | External Database | Details |
|---|---|---|
| Val 516 | Asp 516 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 11 |
| Total formula weight | 622259.1 | |
| All* | Total formula weight | 622259.1 |
