8Y5T
E.coli Transcription translation coupling complex in TTC-B state 5 (subclass 3) containing mRNA with 27-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and fusidic acid
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | 50S ribosomal protein L31 | polymer | 70 | 7887.1 | 1 | UniProt (P0A7M9) Pfam (PF01197) | Escherichia coli K-12 | Large ribosomal subunit protein bL31-A |
| 2 | B (B) | 50S ribosomal protein L32 | polymer | 57 | 6463.4 | 1 | UniProt (P0A7N4) Pfam (PF01783) | Escherichia coli K-12 | Large ribosomal subunit protein bL32 |
| 3 | C (C) | 50S ribosomal protein L33 | polymer | 55 | 6388.6 | 1 | UniProt (P0A7N9) Pfam (PF00471) | Escherichia coli K-12 | Large ribosomal subunit protein bL33 |
| 4 | D (D) | 50S ribosomal protein L34 | polymer | 46 | 5397.5 | 1 | UniProt (P0A7P5) Pfam (PF00468) | Escherichia coli K-12 | Large ribosomal subunit protein bL34 |
| 5 | E (E) | 50S ribosomal protein L35 | polymer | 65 | 7313.0 | 1 | UniProt (P0A7Q1) Pfam (PF01632) | Escherichia coli K-12 | Large ribosomal subunit protein bL35,Ribosomal protein A |
| 6 | F (F) | 50S ribosomal protein L36 | polymer | 38 | 4377.4 | 1 | UniProt (P0A7Q6) Pfam (PF00444) | Escherichia coli K-12 | Large ribosomal subunit protein bL36-A,Ribosomal protein B |
| 7 | G (G) | 30S ribosomal protein S2 | polymer | 241 | 26781.7 | 1 | UniProt (P0A7V0) Pfam (PF00318) | Escherichia coli K-12 | Small ribosomal subunit protein uS2 |
| 8 | H (H) | 30S ribosomal protein S3 | polymer | 233 | 26031.3 | 1 | UniProt (P0A7V3) Pfam (PF07650) Pfam (PF00189) | Escherichia coli K-12 | Small ribosomal subunit protein uS3 |
| 9 | I (I) | 30S ribosomal protein S4 | polymer | 206 | 23514.2 | 1 | UniProt (P0A7V8) Pfam (PF00163) Pfam (PF01479) | Escherichia coli K-12 | Small ribosomal subunit protein uS4 |
| 10 | J (J) | 30S ribosomal protein S5 | polymer | 167 | 17629.4 | 1 | UniProt (P0A7W1) Pfam (PF00333) Pfam (PF03719) | Escherichia coli K-12 | Small ribosomal subunit protein uS5 |
| 11 | K (K) | 30S ribosomal protein S6, fully modified isoform | polymer | 135 | 15727.5 | 1 | UniProt (P02358) Pfam (PF01250) | Escherichia coli K-12 | |
| 12 | L (L) | 30S ribosomal protein S7 | polymer | 179 | 20055.2 | 1 | UniProt (P02359) Pfam (PF00177) | Escherichia coli K-12 | Small ribosomal subunit protein uS7 |
| 13 | M (M) | 30S ribosomal protein S8 | polymer | 130 | 14146.6 | 1 | UniProt (P0A7W7) Pfam (PF00410) | Escherichia coli K-12 | Small ribosomal subunit protein uS8 |
| 14 | N (N) | 30S ribosomal protein S9 | polymer | 130 | 14886.3 | 1 | UniProt (P0A7X3) Pfam (PF00380) | Escherichia coli K-12 | Small ribosomal subunit protein uS9 |
| 15 | O (O) | 30S ribosomal protein S10 | polymer | 103 | 11755.6 | 1 | UniProt (P0A7R5) Pfam (PF00338) | Escherichia coli K-12 | Small ribosomal subunit protein uS10,Transcription termination/antitermination protein NusE |
| 16 | P (P) | 30S ribosomal protein S11 | polymer | 129 | 13871.0 | 1 | UniProt (P0A7R9) Pfam (PF00411) | Escherichia coli K-12 | Small ribosomal subunit protein uS11 |
| 17 | Q (Q) | 30S ribosomal protein S12 | polymer | 124 | 13768.2 | 1 | UniProt (P0A7S3) Pfam (PF00164) | Escherichia coli K-12 | Small ribosomal subunit protein uS12 |
| 18 | R (R) | 30S ribosomal protein S13 | polymer | 118 | 13128.5 | 1 | UniProt (P0A7S9) Pfam (PF00416) | Escherichia coli K-12 | Small ribosomal subunit protein uS13 |
| 19 | S (S) | 30S ribosomal protein S14 | polymer | 101 | 11606.6 | 1 | UniProt (P0AG59) Pfam (PF00253) | Escherichia coli K-12 | Small ribosomal subunit protein uS14 |
| 20 | T (T) | 30S ribosomal protein S15 | polymer | 89 | 10290.8 | 1 | UniProt (P0ADZ4) Pfam (PF00312) | Escherichia coli K-12 | Small ribosomal subunit protein uS15 |
| 21 | U (U) | 30S ribosomal protein S16 | polymer | 82 | 9207.6 | 1 | UniProt (P0A7T3) Pfam (PF00886) | Escherichia coli K-12 | Small ribosomal subunit protein bS16 |
| 22 | V (V) | 30S ribosomal protein S17 | polymer | 84 | 9724.5 | 1 | UniProt (P0AG63) Pfam (PF00366) | Escherichia coli K-12 | Small ribosomal subunit protein uS17 |
| 23 | W (W) | 30S ribosomal protein S18 | polymer | 75 | 9005.5 | 1 | UniProt (P0A7T7) Pfam (PF01084) | Escherichia coli K-12 | Small ribosomal subunit protein bS18 |
| 24 | X (X) | 30S ribosomal protein S19 | polymer | 92 | 10455.4 | 1 | UniProt (P0A7U3) Pfam (PF00203) | Escherichia coli K-12 | Small ribosomal subunit protein uS19 |
| 25 | Y (Y) | 30S ribosomal protein S20 | polymer | 87 | 9708.5 | 1 | UniProt (P0A7U7) Pfam (PF01649) | Escherichia coli K-12 | Small ribosomal subunit protein bS20 |
| 26 | Z (Z) | 30S ribosomal protein S21 | polymer | 71 | 8524.0 | 1 | UniProt (P68679) Pfam (PF01165) | Escherichia coli K-12 | Small ribosomal subunit protein bS21 |
| 27 | AA (b) | 50S ribosomal protein L2 | polymer | 273 | 29923.6 | 1 | UniProt (P60422) Pfam (PF00181) Pfam (PF03947) | Escherichia coli K-12 | Large ribosomal subunit protein uL2 |
| 28 | BA (c) | 50S ribosomal protein L3 | polymer | 209 | 22277.5 | 1 | UniProt (P60438) Pfam (PF00297) | Escherichia coli K-12 | Large ribosomal subunit protein uL3 |
| 29 | CA (d) | 50S ribosomal protein L4 | polymer | 201 | 22121.6 | 1 | UniProt (P60723) Pfam (PF00573) | Escherichia coli K-12 | Large ribosomal subunit protein uL4 |
| 30 | DA (e) | 50S ribosomal protein L5 | polymer | 179 | 20333.6 | 1 | UniProt (P62399) Pfam (PF00281) Pfam (PF00673) | Escherichia coli K-12 | Large ribosomal subunit protein uL5 |
| 31 | EA (f) | 50S ribosomal protein L6 | polymer | 177 | 18932.8 | 1 | UniProt (P0AG55) Pfam (PF00347) | Escherichia coli K-12 | Large ribosomal subunit protein uL6 |
| 32 | FA (g) | 50S ribosomal protein L9 | polymer | 149 | 15789.0 | 1 | UniProt (P0A7R1) Pfam (PF01281) Pfam (PF03948) | Escherichia coli K-12 | Large ribosomal subunit protein bL9 |
| 33 | GA (i) | 50S ribosomal protein L11 | polymer | 142 | 14894.4 | 1 | UniProt (P0A7J7) Pfam (PF03946) Pfam (PF00298) | Escherichia coli K-12 | Large ribosomal subunit protein uL11 |
| 34 | HA (j) | 50S ribosomal protein L13 | polymer | 142 | 16050.6 | 1 | UniProt (P0AA10) Pfam (PF00572) | Escherichia coli K-12 | Large ribosomal subunit protein uL13 |
| 35 | IA (k) | 50S ribosomal protein L14 | polymer | 123 | 13565.1 | 1 | UniProt (P0ADY3) Pfam (PF00238) | Escherichia coli K-12 | Large ribosomal subunit protein uL14 |
| 36 | JA (l) | 50S ribosomal protein L15 | polymer | 144 | 15008.5 | 1 | UniProt (P02413) Pfam (PF00828) | Escherichia coli K-12 | |
| 37 | KA (m) | 50S ribosomal protein L16 | polymer | 136 | 15312.3 | 1 | UniProt (P0ADY7) Pfam (PF00252) | Escherichia coli K-12 | Large ribosomal subunit protein uL16 |
| 38 | LA (n) | 50S ribosomal protein L17 | polymer | 127 | 14393.7 | 1 | UniProt (P0AG44) Pfam (PF01196) | Escherichia coli K-12 | Large ribosomal subunit protein bL17 |
| 39 | MA (o) | 50S ribosomal protein L18 | polymer | 117 | 12794.7 | 1 | UniProt (P0C018) Pfam (PF00861) | Escherichia coli K-12 | Large ribosomal subunit protein uL18 |
| 40 | NA (p) | 50S ribosomal protein L19 | polymer | 115 | 13159.3 | 1 | UniProt (P0A7K6) Pfam (PF01245) | Escherichia coli K-12 | Large ribosomal subunit protein bL19 |
| 41 | OA (q) | 50S ribosomal protein L20 | polymer | 118 | 13528.0 | 1 | UniProt (P0A7L3) Pfam (PF00453) | Escherichia coli K-12 | Large ribosomal subunit protein bL20 |
| 42 | PA (r) | 50S ribosomal protein L21 | polymer | 103 | 11586.4 | 1 | UniProt (P0AG48) Pfam (PF00829) | Escherichia coli K-12 | Large ribosomal subunit protein bL21 |
| 43 | QA (s) | 50S ribosomal protein L22 | polymer | 110 | 12253.4 | 1 | UniProt (P61175) Pfam (PF00237) | Escherichia coli K-12 | Large ribosomal subunit protein uL22 |
| 44 | RA (t) | 50S ribosomal protein L23 | polymer | 100 | 11222.2 | 1 | UniProt (P0ADZ0) Pfam (PF00276) | Escherichia coli K-12 | Large ribosomal subunit protein uL23 |
| 45 | SA (u) | 50S ribosomal protein L24 | polymer | 104 | 11339.3 | 1 | UniProt (P60624) Pfam (PF00467) Pfam (PF17136) | Escherichia coli K-12 | Large ribosomal subunit protein uL24 |
| 46 | TA (v) | 50S ribosomal protein L25 | polymer | 94 | 10713.5 | 1 | UniProt (P68919) Pfam (PF01386) | Escherichia coli K-12 | Large ribosomal subunit protein bL25 |
| 47 | UA (w) | 50S ribosomal protein L27 | polymer | 85 | 9146.5 | 1 | UniProt (P0A7L8) Pfam (PF01016) | Escherichia coli K-12 | Large ribosomal subunit protein bL27 |
| 48 | VA (x) | 50S ribosomal protein L28 | polymer | 78 | 9027.6 | 1 | UniProt (P0A7M2) Pfam (PF00830) | Escherichia coli K-12 | Large ribosomal subunit protein bL28 |
| 49 | WA (y) | 50S ribosomal protein L29 | polymer | 63 | 7286.5 | 1 | UniProt (P0A7M6) Pfam (PF00831) | Escherichia coli K-12 | Large ribosomal subunit protein uL29 |
| 50 | XA (z) | 50S ribosomal protein L30 | polymer | 59 | 6554.8 | 1 | UniProt (P0AG51) Pfam (PF00327) | Escherichia coli K-12 | Large ribosomal subunit protein uL30 |
| 51 | YA (1) | 23S rRNA | polymer | 2904 | 941611.4 | 1 | GenBank (1929590828) | Escherichia coli K-12 | |
| 52 | ZA (2) | 5S rRNA | polymer | 120 | 38813.1 | 1 | GenBank (NR_103249) | Escherichia coli K-12 | |
| 53 | AB (3) | 16S rRNA | polymer | 1542 | 499690.0 | 1 | GenBank (1789840096) | Escherichia coli K-12 | |
| 54 | BB (4) | mRNA | polymer | 44 | 13828.8 | 1 | synthetic construct | ||
| 55 | CB (8) | templete DNA strand | polymer | 37 | 11230.2 | 1 | synthetic construct | ||
| 56 | DB (9) | non-templete DNA strand | polymer | 37 | 11473.3 | 1 | synthetic construct | ||
| 57 | EB, FB (A1, A2) | DNA-directed RNA polymerase subunit alpha | polymer | 329 | 36558.7 | 2 | UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) | Escherichia coli K-12 | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 58 | GB (B1) | DNA-directed RNA polymerase subunit beta' | polymer | 1407 | 155366.8 | 1 | UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Escherichia coli K-12 | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 59 | HB (B2) | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V2) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) | Escherichia coli K-12 | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 60 | IB (W0) | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (P0A800) Pfam (PF01192) | Escherichia coli K-12 | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 61 | JB (NA) | Transcription termination/antitermination protein NusA | polymer | 495 | 54932.7 | 1 | UniProt (P0AFF6) Pfam (PF08529) Pfam (PF00575) Pfam (PF13184) Pfam (PF26594) Pfam (PF14520) | Escherichia coli K-12 | N utilization substance protein A,Transcription termination/antitermination L factor |
| 62 | KB (NG) | Transcription termination/antitermination protein NusG | polymer | 181 | 20560.5 | 1 | UniProt (P0AFG0) Pfam (PF02357) Pfam (PF00467) | Escherichia coli K-12 | |
| 63 | LB (5) | tRNA(Phe) | polymer | 76 | 24485.5 | 1 | GenBank (1845258627) | Escherichia coli K-12 | |
| 64 | MB (6) | tRNA(fMet) | polymer | 77 | 24802.8 | 1 | GenBank (1845258627) | Escherichia coli K-12 | |
| 65 | NB (0) | Elongation factor G | polymer | 716 | 79069.7 | 1 | UniProt (P0A6M8) Pfam (PF00009) Pfam (PF03144) Pfam (PF14492) Pfam (PF03764) Pfam (PF00679) | Escherichia coli K-12 | EF-G |
| 66 | OB (B1) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) PubChem (888) | |||
| 67 | PB (0) | FUSIDIC ACID | non-polymer | 516.7 | 1 | Chemie (FUA) PubChem (3443) PubChem (3032280) PubChem (5820788) PubChem (6604483) PubChem (6914278) PubChem (11466473) PubChem (11973017) PubChem (12110530) PubChem (12305061) PubChem (21148423) PubChem (23636770) PubChem (23637137) PubChem (23639198) PubChem (23639553) PubChem (24867849) PubChem (176519951) PubChem (44655892) PubChem (16757684) PubChem (18530307) PubChem (20639055) PubChem (22800304) PubChem (24871020) PubChem (44345525) PubChem (46781674) PubChem (49868174) PubChem (3032525) PubChem (57373456) PubChem (60138401) PubChem (68099750) PubChem (70684533) PubChem (3000226) PubChem (3001077) PubChem (23423) PubChem (244879) PubChem (71315981) PubChem (71317019) PubChem (73952088) PubChem (89877076) PubChem (90660382) PubChem (90697437) PubChem (5280986) PubChem (5288270) PubChem (5353638) PubChem (53911549) PubChem (53911550) PubChem (6604105) PubChem (124291107) PubChem (124900075) PubChem (124900734) PubChem (124900071) PubChem (124900730) PubChem (124921605) PubChem (126963540) PubChem (57312976) PubChem (134694339) PubChem (11957550) PubChem (12305058) PubChem (12305062) PubChem (137703527) PubChem (142165976) PubChem (154790121) PubChem (58872606) PubChem (161670019) PubChem (163002326) PubChem (171153990) PubChem (175673725) PubChem (60138404) PubChem (66593017) PubChem (73759677) PubChem (73952461) PubChem (90660452) PubChem (90660516) PubChem (91122380) PubChem (91450867) PubChem (91873262) PubChem (118855441) PubChem (123332703) PubChem (124291106) PubChem (124833740) PubChem (124833741) PubChem (124867503) PubChem (124900073) PubChem (124900076) PubChem (124900732) PubChem (124900735) PubChem (124921604) PubChem (124932933) PubChem (124932934) PubChem (125032759) PubChem (126968843) PubChem (131707593) PubChem (132342643) PubChem (133688410) PubChem (157247173) PubChem (163002324) PubChem (163002325) PubChem (163002327) PubChem (163002328) PubChem (163002329) PubChem (163002330) PubChem (163002331) PubChem (165429846) PubChem (166948652) PubChem (172012042) | |||
| 68 | QB (0) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 1 | Chemie (GDP) PubChem (136073927) PubChem (135398619) PubChem (135402030) PubChem (135431654) PubChem (135566337) PubChem (135760556) PubChem (136026707) PubChem (136053418) PubChem (136663964) PubChem (136664748) PubChem (136759848) PubChem (136759849) PubChem (136759850) PubChem (136836636) PubChem (136847074) PubChem (172898537) |
Sequence modifications
1: 1 - 2904 (GenBank: 1929590828)
2: 1 - 120 (GenBank: NR_103249)
0: 1 - 704 (UniProt: P0A6M8)
| PDB | External Database | Details |
|---|---|---|
| C 747 | U 1037988 | conflict |
| PDB | External Database | Details |
|---|---|---|
| A 120 | U 120 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Gly 705 | - | expression tag |
| Ser 706 | - | expression tag |
| Ser 707 | - | expression tag |
| Gly 708 | - | expression tag |
| His 709 | - | expression tag |
| His 710 | - | expression tag |
| His 711 | - | expression tag |
| His 712 | - | expression tag |
| His 713 | - | expression tag |
| His 714 | - | expression tag |
| His 715 | - | expression tag |
| His 716 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 66 |
| Total formula weight | 2784912.4 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 984.2 | |
| All* | Total formula weight | 2785896.6 |






