8Y5I
Cryo-EM structure of E.coli spermidine transporter PotD-PotABC in translocation intermidiate state
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D (A, D) | Spermidine/putrescine import ATP-binding protein PotA | polymer | 378 | 43079.1 | 2 | UniProt (P69874) Pfam (PF00005) Pfam (PF08402) | Escherichia coli | |
| 2 | B (B) | Spermidine/putrescine transport system permease protein PotB | polymer | 285 | 32206.1 | 1 | Pfam (PF00528) UniProt (by SIFTS) (P0AFK4) | Escherichia coli | |
| 3 | C (C) | Spermidine/putrescine transport system permease protein PotC | polymer | 264 | 29132.8 | 1 | UniProt (P0AFK6) Pfam (PF00528) | Escherichia coli | |
| 4 | E (E) | Putrescine-binding periplasmic protein | polymer | 348 | 38906.9 | 1 | UniProt (C3TDJ2) Pfam (PF13416) UniProt (by SIFTS) (P0AFK9) | Escherichia coli | |
| 5 | F, H (A, D) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 2 | Chemie (ATP) | |||
| 6 | G, I (A, D) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) |
Sequence modifications
A, D: 1 - 378 (UniProt: P69874)
| PDB | External Database | Details |
|---|---|---|
| Gln 173 | Glu 173 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 186404.2 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 1063.0 | |
| All* | Total formula weight | 187467.1 |
