8Y02
Cryo-EM structure of Short-wave-sensitive opsin 1
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40447.1 | 1 | UniProt (P63096) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 340 | 37416.9 | 1 | UniProt (P62873) Pfam (PF25391) | Homo sapiens (human) | Transducin beta chain 1 |
| 3 | C (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P63212) Pfam (PF00631) | Bos taurus (domestic cattle) | G gamma-I |
| 4 | D (R) | Short-wave-sensitive opsin 1 | polymer | 348 | 39164.8 | 1 | UniProt (P03999) Pfam (PF00001) | Homo sapiens (human) | Blue cone photoreceptor pigment,Blue-sensitive opsin,BOP |
| 5 | E (S) | scFv Recombinant Human Monoclonal Antibody (scFv16) | polymer | 267 | 28668.9 | 1 | Homo sapiens (human) | ||
| 6 | F (R) | RETINAL | non-polymer | 284.4 | 1 | Chemie (RET) PubChem (638015) PubChem (5381765) |
Sequence modifications
A: 1 - 354 (UniProt: P63096)
R: 4 - 348 (UniProt: P03999)
| PDB | External Database | Details |
|---|---|---|
| Cys 47 | Ser 47 | engineered mutation |
| Thr 202 | Gly 202 | engineered mutation |
| Ala 203 | Gly 203 | engineered mutation |
| Ala 245 | Glu 245 | engineered mutation |
| Ser 326 | Ala 326 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Arg 2 | - | expression tag |
| Lys 3 | - | expression tag |
| Gln 110 | Glu 107 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 153559.0 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 284.4 | |
| All* | Total formula weight | 153843.4 |
