8Y00
Structure of a xylanase Xyl-1 M4 mutant E175A in complex with xylotriose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Endo-1,4-beta-xylanase | polymer | 201 | 21502.8 | 4 | UniProt (Q0CMZ1) Pfam (PF00457) | Aspergillus terreus NIH2624 | xylotriose |
| 2 | E, H (E, H) | beta-D-xylopyranose-(1-4)-beta-D-xylopyranose | branched | 282.2 | 2 | In PDB GlyTouCan (G87728WL) | |||
| 3 | F, G (F, G) | beta-D-xylopyranose-(1-4)-beta-D-xylopyranose-(1-4)-beta-D-xylopyranose | branched | 414.4 | 2 | In PDB GlyTouCan (G87429QT) | |||
| 4 | I, J, L, M, N... (A, B, C) | ZINC ION | non-polymer | 65.4 | 6 | Chemie (ZN) | |||
| 5 | K, P (A, D) | beta-D-xylopyranose | non-polymer | 150.1 | 2 | Chemie (XYP) | |||
| 6 | Q, R, S, T (A, B, C, D) | water | water | 18.0 | 700 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 2 - 189 (UniProt: Q0CMZ1)
| PDB | External Database | Details |
|---|---|---|
| Gly -11 | - | expression tag |
| Ala -10 | - | expression tag |
| Gly -9 | - | expression tag |
| Ala -8 | - | expression tag |
| Gly -7 | - | expression tag |
| Ala -6 | - | expression tag |
| Gly -5 | - | expression tag |
| Ala -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Met 0 | - | expression tag |
| Glu 1 | - | expression tag |
| Pro 31 | Asp 62 | engineered mutation |
| Gln 34 | Ala 65 | engineered mutation |
| Gln 118 | Lys 149 | engineered mutation |
| His 168 | Ala 199 | engineered mutation |
| Ala 176 | Glu 207 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 86011.1 | |
| Branched | Number of molecules | 4 |
| Total formula weight | 1393.2 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 692.7 | |
| All* | Total formula weight | 88097.0 |
