8XY0

Activity-stability trade-off observed in variants at position 315 of the GH10 xylanase XynR

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Endo-1,4-beta-xylanase A	polymer	360	42058.2	1	UniProt (P07528) Pfam (PF00331) In PDB	Bacillus sp. TAR1	Xylanase A,1,4-beta-D-xylan xylanohydrolase A
2	A	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
3	A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	3	Chemie (PEG)
4		water	water		18.0	336	Chemie (HOH)

Sequence modifications

A: 1 - 351 (UniProt: P07528)

PDB	External Database	Details
Met 0	-	initiating methionine
Gln 315	Thr 360	engineered mutation
Leu 352	-	expression tag
Glu 353	-	expression tag
His 354	-	expression tag
His 355	-	expression tag
His 356	-	expression tag
His 357	-	expression tag
His 358	-	expression tag
His 359	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	42058.2
Non-Polymers*	Number of molecules	4
	Total formula weight	358.4
All*	Total formula weight	42416.6

*Water molecules are not included.