8XY0
Activity-stability trade-off observed in variants at position 315 of the GH10 xylanase XynR
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A (A) | Endo-1,4-beta-xylanase A | polymer | 360 | 42058.2 | 1 | UniProt (P07528) Pfam (PF00331) | Bacillus sp. TAR1 | Xylanase A,1,4-beta-D-xylan xylanohydrolase A |
2 | B (A) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
3 | C, D, E (A) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 3 | Chemie (PEG) | |||
4 | F (A) | water | water | 18.0 | 336 | Chemie (HOH) |
Sequence modifications
A: 1 - 351 (UniProt: P07528)
PDB | External Database | Details |
---|---|---|
Met 0 | - | initiating methionine |
Gln 315 | Thr 360 | engineered mutation |
Leu 352 | - | expression tag |
Glu 353 | - | expression tag |
His 354 | - | expression tag |
His 355 | - | expression tag |
His 356 | - | expression tag |
His 357 | - | expression tag |
His 358 | - | expression tag |
His 359 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 42058.2 | |
Non-Polymers* | Number of molecules | 4 |
Total formula weight | 358.4 | |
All* | Total formula weight | 42416.6 |