8XWU
Crystal structure of human 8-oxoguanine glycosylase K249H mutant bound to the reaction intermediate derived from the crystal soaked into the solution at pH 4.0 under 277 K for 24 hourss
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (C) | DNA (5'-D(*AP*GP*CP*GP*TP*CP*CP*A)-3') | polymer | 8 | 2411.6 | 1 | Homo sapiens | ||
| 2 | B (D) | DNA (5'-D(P*GP*GP*TP*AP*GP*AP*CP*CP*TP*GP*GP*AP*CP*GP*C)-3') | polymer | 15 | 4635.0 | 1 | Homo sapiens | ||
| 3 | C (A) | N-glycosylase/DNA lyase | polymer | 336 | 37700.6 | 1 | UniProt (O15527) Pfam (PF07934) Pfam (PF00730) | Homo sapiens (human) | |
| 4 | D (B) | DNA (5'-D(P*GP*TP*CP*TP*AP*CP*C)-3') | polymer | 7 | 2073.4 | 1 | Homo sapiens | ||
| 5 | E (C) | [(2~{R},3~{S},5~{S})-5-[2-azanyl-6,8-bis(oxidanylidene)-1,7-dihydropurin-9-yl]-2,3,5-tris(oxidanyl)pentyl] dihydrogen phosphate | non-polymer | 381.2 | 1 | Chemie (A1LXK) | |||
| 6 | F, G (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) PubChem (888) | |||
| 7 | H, I, J (A, B) | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) PubChem (753) | |||
| 8 | K, L, M, N (C, D, A, B) | water | water | 18.0 | 378 | Chemie (HOH) |
Sequence modifications
A: 12 - 345 (UniProt: O15527)
| PDB | External Database | Details |
|---|---|---|
| Gly 10 | - | expression tag |
| Pro 11 | - | expression tag |
| His 249 | Lys 249 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 46820.6 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 706.1 | |
| All* | Total formula weight | 47526.8 |
