8XVR
Crystal structure of inulosucrase from Lactobacillus reuteri 121 mutant R544W
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Glycoside hydrolase family 68 protein | polymer | 588 | 65235.4 | 4 | UniProt (A0A6N1ER42) UniProt (by SIFTS) (A0A7X2FZN4) | Limosilactobacillus reuteri | |
| 2 | E, F, JA, KA, N... (A, D, B, C) | CALCIUM ION | non-polymer | 40.1 | 8 | Chemie (CA) | |||
| 3 | AA, BA, CA, DA, EA... (C, A, D, B) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 24 | Chemie (EDO) | |||
| 4 | HA, IA, L, PA, W (C, A, D, B) | GLYCEROL | non-polymer | 92.1 | 5 | Chemie (GOL) | |||
| 5 | M (A) | PENTAETHYLENE GLYCOL | non-polymer | 238.3 | 1 | Chemie (1PE) | |||
| 6 | QA (D) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
| 7 | RA, SA, TA, UA (A, B, C, D) | water | water | 18.0 | 995 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 121 - 701 (UniProt: A0A6N1ER42)
| PDB | External Database | Details |
|---|---|---|
| Met 120 | - | initiating methionine |
| Pro 300 | Leu 300 | conflict |
| Trp 544 | Arg 544 | engineered mutation |
| His 702 | - | expression tag |
| His 703 | - | expression tag |
| His 704 | - | expression tag |
| His 705 | - | expression tag |
| His 706 | - | expression tag |
| His 707 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 260941.5 | |
| Non-Polymers* | Number of molecules | 39 |
| Total formula weight | 2615.1 | |
| All* | Total formula weight | 263556.6 |
