8XVI

Cryo-EM structure of ETAR bound with Endothelin1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Isoform Gnas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	261	30464.3	1		Homo sapiens (human)
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	346	38045.6	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
3	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
4	N	Nanobody 35	polymer	157	17057.3	1		Lama glama
5	R	Endoglucanase H,Endothelin-1 receptor	polymer	683	77879.4	1	UniProt (P16218) UniProt (P25101) Pfam (PF00001) Pfam (PF02156) In PDB	Acetivibrio thermocellus ATCC 27405	Cellulase H,Endo-1,4-beta-glucanase H,EgH,Endothelin receptor type A,ET-A,ETA-R,hET-AR
6	T	Endothelin-1	polymer	21	2498.0	1	UniProt (P05305) Pfam (PF00322) In PDB	Homo sapiens (human)	ET-1

Sequence modifications

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Ile -5	-	expression tag
Gly -4	-	expression tag
Arg -3	-	expression tag
Ala -2	-	expression tag
Arg -1	-	expression tag
Gly 0	-	expression tag
Phe 1	-	expression tag

R: -229 - 49 (UniProt: P16218)

PDB	External Database	Details
Met -259	-	initiating methionine
Lys -258	-	expression tag
Thr -257	-	expression tag
Ile -256	-	expression tag
Ile -255	-	expression tag
Ala -254	-	expression tag
Leu -253	-	expression tag
Ser -252	-	expression tag
Tyr -251	-	expression tag
Ile -250	-	expression tag
Phe -249	-	expression tag
Cys -248	-	expression tag
Leu -247	-	expression tag
Val -246	-	expression tag
Phe -245	-	expression tag
Ala -244	-	expression tag
Asp -243	-	expression tag
Tyr -242	-	expression tag
Lys -241	-	expression tag
Asp -240	-	expression tag
Asp -239	-	expression tag
Asp -238	-	expression tag
Asp -237	-	expression tag
Ala -236	-	expression tag
Gly -235	-	expression tag
Arg -234	-	expression tag
Ala -233	-	expression tag
Met -232	-	expression tag
Ala -231	-	expression tag
Ser -230	-	expression tag
Ala -124	Glu 131	conflict

R: 50 - 405 (UniProt: P25101)

PDB	External Database	Details
Leu 406	-	expression tag
Glu 407	-	expression tag
Val 408	-	expression tag
Leu 409	-	expression tag
Phe 410	-	expression tag
Gln 411	-	expression tag
Gly 412	-	expression tag
Pro 413	-	expression tag
His 414	-	expression tag
His 415	-	expression tag
His 416	-	expression tag
His 417	-	expression tag
His 418	-	expression tag
His 419	-	expression tag
His 420	-	expression tag
His 421	-	expression tag
His 422	-	expression tag
His 423	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	173805.7
All*	Total formula weight	173805.7

*Water molecules are not included.