8XRZ

Crystal structure of a novel PU plastic degradation enzyme with ligand from Thermaerobacter marianensis

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Carboxylic ester hydrolase	polymer	527	56375.7	4	UniProt (E6SHQ4) Pfam (PF00135)	Thermaerobacter marianensis (strain ATCC 700841 / DSM 12885 / JCM 10246 / 7p75a)
2	E (A)	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
3	F (C)	4-oxidanylbutyl ~{N}-[4-[(4-aminophenyl)methyl]phenyl]carbamate	non-polymer		314.4	1	Chemie (A1D5F)
4	G, H, I, J (A, B, C, D)	water	water		18.0	469	Chemie (HOH)

Sequence modifications

A, B, C, D: 17 - 520 (UniProt: E6SHQ4)

PDB	External Database	Details
Met -6	-	initiating methionine
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Glu 1	-	expression tag
Asn 2	-	expression tag
Leu 3	-	expression tag
Tyr 4	-	expression tag
Phe 5	-	expression tag
Gln 6	-	expression tag
Gly 7	-	expression tag
Ala 8	-	expression tag
Gly 9	-	expression tag
Ala 10	-	expression tag
Gly 11	-	expression tag
Ala 12	-	expression tag
Gly 13	-	expression tag
Ala 14	-	expression tag
Gly 15	-	expression tag
Ala 16	-	expression tag
Ala 215	Ser 199	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	225502.6
Non-Polymers*	Number of molecules	2
	Total formula weight	410.4
All*	Total formula weight	225913.1

*Water molecules are not included.