8XQN

Structure of human class T GPCR TAS2R14-DNGi complex with Aristolochic acid A.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	370	42455.2	1	UniProt (P63096) In PDB	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	366	39728.4	1	UniProt (P62873) In PDB	Homo sapiens (human)	Transducin beta chain 1
3	C	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) In PDB	Homo sapiens (human)	G gamma-I
4	S	scFv16	polymer	286	30668.2	1		Homo sapiens (human)
5	R	Exo-alpha-sialidase,Taste receptor type 2 member 14,LgBiT	polymer	990	110840.0	1	UniProt (Q59310) UniProt (Q9NYV8) In PDB	Clostridium perfringens	Bitter taste receptor T2R14, T2R14,Taste receptor family B member 1,TRB1
6	R	8-methoxy-6-nitro-naphtho[1,2-e][1,3]benzodioxole-5-carboxylic acid	non-polymer		341.3	1	Chemie (GOQ)
7	R	CHOLESTEROL	non-polymer		386.7	1	Chemie (CLR)

Sequence modifications

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Met -15	-	initiating methionine
Asp -14	-	expression tag
Tyr -13	-	expression tag
Lys -12	-	expression tag
Asp -11	-	expression tag
Asp -10	-	expression tag
Asp -9	-	expression tag
Asp -8	-	expression tag
Lys -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Ser 0	-	expression tag
Ala 203	Gly 203	conflict
Ser 326	Ala 326	conflict

B: 1 - 340 (UniProt: P62873)

PDB	External Database	Details
Gly 341	-	expression tag
Ser 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Gly 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Ser 354	-	expression tag
Gly 355	-	expression tag
Val 356	-	expression tag
Ser 357	-	expression tag
Gly 358	-	expression tag
Trp 359	-	expression tag
Arg 360	-	expression tag
Leu 361	-	expression tag
Phe 362	-	expression tag
Lys 363	-	expression tag
Lys 364	-	expression tag
Ile 365	-	expression tag
Ser 366	-	expression tag

R: -455 - -4 (UniProt: Q59310)

PDB	External Database	Details
Met -499	-	initiating methionine
Lys -498	-	expression tag
Thr -497	-	expression tag
Ile -496	-	expression tag
Ile -495	-	expression tag
Ala -494	-	expression tag
Leu -493	-	expression tag
Ser -492	-	expression tag
Tyr -491	-	expression tag
Ile -490	-	expression tag
Phe -489	-	expression tag
Cys -488	-	expression tag
Leu -487	-	expression tag
Val -486	-	expression tag
Phe -485	-	expression tag
Ala -484	-	expression tag
Asp -483	-	expression tag
Tyr -482	-	expression tag
Lys -481	-	expression tag
Asp -480	-	expression tag
Asp -479	-	expression tag
Asp -478	-	expression tag
Asp -477	-	expression tag
Ala -476	-	expression tag
His -475	-	expression tag
His -474	-	expression tag
His -473	-	expression tag
His -472	-	expression tag
His -471	-	expression tag
His -470	-	expression tag
His -469	-	expression tag
His -468	-	expression tag
His -467	-	expression tag
His -466	-	expression tag
Glu -465	-	expression tag
Asn -464	-	expression tag
Leu -463	-	expression tag
Tyr -462	-	expression tag
Phe -461	-	expression tag
Gln -460	-	expression tag
Ser -459	-	expression tag
Gly -458	-	expression tag
Arg -457	-	expression tag
Ala -456	-	expression tag
Ser -305	Gly 393	conflict
Gly -3	-	linker
Ser -2	-	linker
Ala -1	-	linker
Gly 0	-	linker
Ser 1	-	linker

R: 2 - 317 (UniProt: Q9NYV8)
R: 318 - 490 (PDB: 8XQN)

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	231553.0
Non-Polymers*	Number of molecules	2
	Total formula weight	727.9
All*	Total formula weight	232280.9

*Water molecules are not included.