8XQE

Cryo-EM structure of human dimeric APJR-Gi complex with apelin-13.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	G protein subunit alpha i1	polymer	356	40616.2	1	UniProt (A0A096MZM0)	Homo sapiens (human)
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	340	37416.9	1	UniProt (P62873)	Homo sapiens (human)	Transducin beta chain 1,G protein beta subunit
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768)	Homo sapiens (human)	G gamma-I
4	D, G (R, Q)	Soluble cytochrome b562,Apelin receptor	polymer	477	54312.6	2	UniProt (P0ABE7) UniProt (P35414)	Homo sapiens (human)	Cytochrome b-562,Angiotensin receptor-like 1,G-protein coupled receptor APJ,G-protein coupled receptor HG11
5	E (S)	scFv16	polymer	259	27784.9	1		Homo sapiens
6	F (D)	Apelin-13	polymer	13	1554.9	1	UniProt (Q9ULZ1)	Homo sapiens (human)

Sequence modifications

A: -1 - 354 (UniProt: A0A096MZM0)

PDB	External Database	Details
Ser 0	Asn 68	conflict
Asn 47	Ser 115	conflict
Ala 203	Gly 271	conflict
Ser 326	Ala 394	conflict

R, Q: -104 - 0 (UniProt: P0ABE7)

PDB	External Database	Details
Met -146	-	initiating methionine
Lys -145	-	expression tag
Thr -144	-	expression tag
Ile -143	-	expression tag
Ile -142	-	expression tag
Ala -141	-	expression tag
Leu -140	-	expression tag
Ser -139	-	expression tag
Tyr -138	-	expression tag
Ile -137	-	expression tag
Phe -136	-	expression tag
Cys -135	-	expression tag
Leu -134	-	expression tag
Val -133	-	expression tag
Phe -132	-	expression tag
Ala -131	-	expression tag
Asp -130	-	expression tag
Tyr -129	-	expression tag
Lys -128	-	expression tag
Asp -127	-	expression tag
Asp -126	-	expression tag
Asp -125	-	expression tag
Asp -124	-	expression tag
Lys -123	-	expression tag
His -122	-	expression tag
His -121	-	expression tag
His -120	-	expression tag
His -119	-	expression tag
His -118	-	expression tag
His -117	-	expression tag
His -116	-	expression tag
His -115	-	expression tag
His -114	-	expression tag
His -113	-	expression tag
Leu -112	-	expression tag
Glu -111	-	expression tag
Val -110	-	expression tag
Leu -109	-	expression tag
Phe -108	-	expression tag
Gln -107	-	expression tag
Gly -106	-	expression tag
Pro -105	-	expression tag
Trp -98	Met 29	conflict
Ile -3	His 124	conflict

R, Q: 1 - 330 (UniProt: P35414)

PDB	External Database	Details
Leu 1	Met 1	conflict

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	223859.2
All*	Total formula weight	223859.2

*Water molecules are not included.