8XOP

Cryo-EM structure of ClpP1P2 in complex with ADEP1 from Streptomyces hawaiiensis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	ATP-dependent Clp protease proteolytic subunit	polymer	210	22632.6	7	UniProt (A0A5B9BGY8) Pfam (PF00574) In PDB	Streptomyces hawaiiensis	ClpP1, Endopeptidase Clp
2	H, I, J, K, L...	ATP-dependent Clp protease proteolytic subunit	polymer	220	24058.2	7	UniProt (A0A5B9BIX9) Pfam (PF00574) In PDB	Streptomyces hawaiiensis	ClpP2, Endopeptidase Clp
3	O, P, Q, R, S...	ADEP1	polymer	7	736.9	14	BIRD (PRD_000503)	synthetic construct

Sequence modifications

A, B, C, D, E, F, G: 31 - 219 (UniProt: A0A5B9BGY8)

PDB	External Database	Details
Met 10	-	initiating methionine
Gly 11	-	expression tag
Ser 12	-	expression tag
Ser 13	-	expression tag
His 14	-	expression tag
His 15	-	expression tag
His 16	-	expression tag
His 17	-	expression tag
His 18	-	expression tag
His 19	-	expression tag
Ser 20	-	expression tag
Ser 21	-	expression tag
Gly 22	-	expression tag
Leu 23	-	expression tag
Val 24	-	expression tag
Pro 25	-	expression tag
Arg 26	-	expression tag
Gly 27	-	expression tag
Ser 28	-	expression tag
His 29	-	expression tag
Met 30	-	expression tag
Ala 113	Ser 113	engineered mutation

H, I, J, K, L, M, N: 52 - 235 (UniProt: A0A5B9BIX9)

PDB	External Database	Details
Met 16	-	initiating methionine
Gly 17	-	expression tag
Ser 18	-	expression tag
Ser 19	-	expression tag
His 20	-	expression tag
His 21	-	expression tag
His 22	-	expression tag
His 23	-	expression tag
His 24	-	expression tag
His 25	-	expression tag
Ser 26	-	expression tag
Ser 27	-	expression tag
Gly 28	-	expression tag
Leu 29	-	expression tag
Val 30	-	expression tag
Pro 31	-	expression tag
Arg 32	-	expression tag
Gly 33	-	expression tag
Ser 34	-	expression tag
His 35	-	expression tag
Met 36	-	expression tag
Ala 37	-	expression tag
Ser 38	-	expression tag
Met 39	-	expression tag
Thr 40	-	expression tag
Gly 41	-	expression tag
Gly 42	-	expression tag
Gln 43	-	expression tag
Gln 44	-	expression tag
Met 45	-	expression tag
Gly 46	-	expression tag
Arg 47	-	expression tag
Gly 48	-	expression tag
Ser 49	-	expression tag
Glu 50	-	expression tag
Tyr 51	-	expression tag
Ala 131	Ser 131	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	28
	Total formula weight	337151.9
All*	Total formula weight	337151.9

*Water molecules are not included.