8XON

Cryo-EM structure of the ClpC1:ClpP1P2 degradation complex in Streptomyces hawaiiensis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	S, R, Q, P, O...	NDP-hexose 4-ketoreductase	polymer	696	77312.1	6	UniProt (A0A6G5RIJ6) Pfam (PF00004) Pfam (PF17871) Pfam (PF07724) Pfam (PF10431) In PDB	Streptomyces hawaiiensis
2	X	casein	polymer	24	2060.5	1		Bos taurus
3	A, B, C, D, E...	ATP-dependent Clp protease proteolytic subunit	polymer	226	24216.3	7	UniProt (A0A5B9BGY8) Pfam (PF00574) In PDB	Streptomyces hawaiiensis	Endopeptidase Clp
4	H, I, J, K, L...	ATP-dependent Clp protease proteolytic subunit	polymer	207	22807.8	7	UniProt (A0A5B9BIX9) Pfam (PF00574) In PDB	Streptomyces hawaiiensis	Endopeptidase Clp
5	R, Q, P, O, T...	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	8	Chemie (ATP)
6	R, Q, P, T, S	MAGNESIUM ION	non-polymer		24.3	7	Chemie (MG)
7	Q, P, O, T	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	4	Chemie (ADP)

Sequence modifications

S, R, Q, P, O, T: 157 - 816 (UniProt: A0A6G5RIJ6)

PDB	External Database	Details
Met 121	-	initiating methionine
Gly 122	-	expression tag
Ser 123	-	expression tag
Ser 124	-	expression tag
His 125	-	expression tag
His 126	-	expression tag
His 127	-	expression tag
His 128	-	expression tag
His 129	-	expression tag
His 130	-	expression tag
Ser 131	-	expression tag
Ser 132	-	expression tag
Gly 133	-	expression tag
Leu 134	-	expression tag
Val 135	-	expression tag
Pro 136	-	expression tag
Arg 137	-	expression tag
Gly 138	-	expression tag
Ser 139	-	expression tag
His 140	-	expression tag
Met 141	-	expression tag
Ala 142	-	expression tag
Ser 143	-	expression tag
Met 144	-	expression tag
Thr 145	-	expression tag
Gly 146	-	expression tag
Gly 147	-	expression tag
Gln 148	-	expression tag
Gln 149	-	expression tag
Met 150	-	expression tag
Gly 151	-	expression tag
Arg 152	-	expression tag
Gly 153	-	expression tag
Ser 154	-	expression tag
Glu 155	-	expression tag
Phe 156	-	expression tag
Ala 284	Glu 284	engineered mutation
Ala 440	Phe 440	engineered mutation
Ala 622	Glu 622	engineered mutation

A, B, C, D, E, F, G: 30 - 219 (UniProt: A0A5B9BGY8)

PDB	External Database	Details
Met -6	-	initiating methionine
Gly -5	-	expression tag
Ser -4	-	expression tag
Ser -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
His 2	-	expression tag
His 3	-	expression tag
Ser 4	-	expression tag
Ser 5	-	expression tag
Gly 6	-	expression tag
Leu 7	-	expression tag
Val 8	-	expression tag
Pro 9	-	expression tag
Arg 10	-	expression tag
Gly 11	-	expression tag
Ser 12	-	expression tag
His 13	-	expression tag
Met 14	-	expression tag
Ala 15	-	expression tag
Ser 16	-	expression tag
Met 17	-	expression tag
Thr 18	-	expression tag
Gly 19	-	expression tag
Gly 20	-	expression tag
Gln 21	-	expression tag
Gln 22	-	expression tag
Met 23	-	expression tag
Gly 24	-	expression tag
Arg 25	-	expression tag
Gly 26	-	expression tag
Ser 27	-	expression tag
Glu 28	-	expression tag
Phe 29	-	expression tag
Ala 113	Ser 113	engineered mutation

H, I, J, K, L, M, N: 50 - 235 (UniProt: A0A5B9BIX9)

PDB	External Database	Details
Met 29	-	initiating methionine
Gly 30	-	expression tag
Ser 31	-	expression tag
Ser 32	-	expression tag
His 33	-	expression tag
His 34	-	expression tag
His 35	-	expression tag
His 36	-	expression tag
His 37	-	expression tag
His 38	-	expression tag
Ser 39	-	expression tag
Ser 40	-	expression tag
Gly 41	-	expression tag
Leu 42	-	expression tag
Val 43	-	expression tag
Pro 44	-	expression tag
Arg 45	-	expression tag
Gly 46	-	expression tag
Ser 47	-	expression tag
His 48	-	expression tag
Met 49	-	expression tag
Ala 131	Ser 131	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	21
	Total formula weight	795102.2
Non-Polymers*	Number of molecules	19
	Total formula weight	5936.4
All*	Total formula weight	801038.5

*Water molecules are not included.