8XOH

Cryo-EM structure of GPR30-Gq complex structure in the presence of E2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(q) subunit alpha-q	polymer	361	41632.2	1	Pfam (PF00503) UniProt (by SIFTS) (P63092) UniProt (by SIFTS) (P63096) In PDB	Homo sapiens
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens	Transducin beta chain 1
3	E	scFv16	polymer	247	26293.3	1		synthetic construct
4	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens	G gamma-I
5	R	G-protein coupled estrogen receptor 1	polymer	375	42291.5	1	UniProt (Q99527) Pfam (PF00001) In PDB	Homo sapiens	Chemoattractant receptor-like 2,Flow-induced endothelial G-protein coupled receptor 1,FEG-1,G protein-coupled estrogen receptor 1,G-protein coupled receptor 30,GPCR-Br,IL8-related receptor DRY12,Lymphocyte-derived G-protein coupled receptor,LYGPR,Membrane estrogen receptor,mER

Sequence modifications

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -10	-	initiating methionine
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	156822.5
All*	Total formula weight	156822.5

*Water molecules are not included.