8XML
Cryo-EM structure of the Apo CCR8-Gi complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (R) | Soluble cytochrome b562,C-C chemokine receptor type 8 | polymer | 461 | 52685.4 | 1 | UniProt (P0ABE7) UniProt (P51685) Pfam (PF07361) Pfam (PF00001) | Escherichia coli | Cytochrome b-562,C-C CKR-8,CC-CKR-8,CCR-8,CC chemokine receptor CHEMR1,CMKBRL2,Chemokine receptor-like 1,CKR-L1,GPR-CY6,GPRCY6,TER1 |
| 2 | B (A) | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40447.1 | 1 | UniProt (P63096) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
| 3 | C (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 340 | 37416.9 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (human) | Transducin beta chain 1,G protein beta subunit |
| 4 | D (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 5 | E (S) | scFv16 | polymer | 267 | 28668.9 | 1 | Homo sapiens |
Sequence modifications
R: -105 - -1 (UniProt: P0ABE7)
R: 1 - 355 (UniProt: P51685)
A: 1 - 354 (UniProt: P63096)
| PDB | External Database | Details |
|---|---|---|
| Trp -99 | Met 29 | conflict |
| Ile -4 | His 124 | conflict |
| Leu 0 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Trp 122 | Phe 122 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Cys 47 | Ser 47 | engineered mutation |
| Thr 202 | Gly 202 | engineered mutation |
| Ala 203 | Gly 203 | engineered mutation |
| Ala 245 | Glu 245 | engineered mutation |
| Ser 326 | Ala 326 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 167079.5 | |
| All* | Total formula weight | 167079.5 |
