8XLD
Structure of the GFP:GFP-nanobody complex from Biortus.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Fluorescent protein | polymer | 219 | 24639.6 | 1 | UniProt (A0A8S0GSD4) Pfam (PF01353) | Cytaeis uchidae | |
| 2 | B (B) | Nanobody(Staygold-S2G10)-Nanobody(Staygold-S4F1) | polymer | 289 | 29214.9 | 1 | synthetic construct | ||
| 3 | C, E, F (A, B) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 3 | Chemie (EDO) | |||
| 4 | D (A) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 5 | G, H (A, B) | water | water | 18.0 | 247 | Chemie (HOH) |
Sequence modifications
A: 1 - 217 (UniProt: A0A8S0GSD4)
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Cr2 58 | Gly 57 | chromophore |
| Cr2 58 | Tyr 58 | chromophore |
| Cr2 58 | Gly 59 | chromophore |
| Ala 137 | Asn 137 | engineered mutation |
| Ser 140 | Gln 140 | engineered mutation |
| Ala 168 | Val 168 | conflict |
| Phe 187 | Tyr 187 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 53854.5 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 251.6 | |
| All* | Total formula weight | 54106.1 |
