8XK9

ternary complex of DNA polymerase SFM4-3 recognizing C2 methyoxy nucleotide

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (B, E)	DNA (5'-D(*GP*AP*CP*CP*AP*CP*GP*GP*CP*GP*CP*(DOC))-3')	polymer	12	3617.4	2		synthetic construct
2	B, E (C, F)	DNA (5'-D(*AP*AP*CP*GP*GP*CP*GP*CP*CP*GP*TP*GP*GP*TP*C)-R(P*(OMG))-3')	polymer	16	4970.2	2		synthetic construct
3	C, F (A, D)	DNA polymerase I, thermostable	polymer	539	60796.8	2	UniProt (P19821) Pfam (PF09281) Pfam (PF00476) UniProt (by SIFTS) (P52028)	Thermus aquaticus	Taq polymerase 1
4	G, H, I, M, N... (B, A, E, D)	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG)
5	J, P (A, D)	[[(2~{R},3~{R},4~{R},5~{R})-5-(2-azanyl-6-oxidanylidene-1~{H}-purin-9-yl)-4-methoxy-3-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate	non-polymer		537.2	2	Chemie (A1LWE)
6	K, Q (A, D)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
7	L, R (A, D)	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
8	S, T, U, V, W... (B, C, A, E, F...)	water	water		18.0	537	Chemie (HOH)

Sequence modifications

A, D: 294 - 832 (UniProt: P19821)

PDB	External Database	Details
Met 294	Leu 294	conflict
Ala 518	Val 518	conflict
Ser 583	Asn 583	conflict
Glu 614	Ile 614	conflict
Gly 615	Glu 615	conflict
Asn 655	Asp 655	conflict
Lys 681	Glu 681	conflict
Gln 742	Glu 742	conflict
Arg 747	Met 747	conflict

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	138768.9
Non-Polymers*	Number of molecules	12
	Total formula weight	1622.4
All*	Total formula weight	140391.3

*Water molecules are not included.