8XK7

binary complex of DNA polymerase SFM4-3 recognizing C2 methyoxy nucleotide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA polymerase I, thermostable	polymer	554	62465.7	1	UniProt (P19821)	Thermus aquaticus	Taq polymerase 1
2	B (B)	DNA(5'-D(*AP*AP*AP*CP*GP*GP*CP*GP*CP*CP*GP*TP*GP*GP*TP*CP*OMG)-3')	polymer	17	5283.4	1		synthetic construct
3	C (C)	DNA(5'-D(*GP*AP*CP*CP*AP*CP*GP*GP*CP*GP*CP*CP*OMG)-3')	polymer	13	3992.6	1		synthetic construct
4	D, E (A)	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
5	F, G, H (A)	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
6	I (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
7	J, K, L (A, B, C)	water	water		18.0	346	Chemie (HOH)

Sequence modifications

A: 294 - 832 (UniProt: P19821)

PDB	External Database	Details
Met 294	Leu 294	conflict
Ala 518	Val 518	conflict
Ser 583	Asn 583	conflict
Glu 614	Ile 614	conflict
Gly 615	Glu 615	conflict
Asn 655	Asp 655	conflict
Lys 681	Glu 681	conflict
Gln 742	Glu 742	conflict
Arg 747	Met 747	conflict
Ala 833	-	expression tag
Ala 834	-	expression tag
Lys 835	-	expression tag
Leu 836	-	expression tag
Ala 837	-	expression tag
Ala 838	-	expression tag
Ala 839	-	expression tag
Leu 840	-	expression tag
Glu 841	-	expression tag
His 842	-	expression tag
His 843	-	expression tag
His 844	-	expression tag
His 845	-	expression tag
His 846	-	expression tag
His 847	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	71741.8
Non-Polymers*	Number of molecules	6
	Total formula weight	584.3
All*	Total formula weight	72326.0

*Water molecules are not included.