8XJY

Cryo-EM structure of colibactin assembly line polyketide synthase ClbI KS-AT didomain crosslinked with ClbI ACP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Polyketide synthase	polymer	921	99125.7	2	UniProt (Q0P7J9)	Escherichia coli	Putative polyketide synthase
2	C, D (C, D)	Polyketide synthase	polymer	141	15369.2	2	UniProt (Q0P7J9)	Escherichia coli	Putative polyketide synthase

Sequence modifications

A, B: 1 - 895 (UniProt: Q0P7J9)

PDB	External Database	Details
Lys 896	-	expression tag
Leu 897	-	expression tag
Ser 898	-	expression tag
Ser 899	-	expression tag
Gly 900	-	expression tag
Leu 901	-	expression tag
Glu 902	-	expression tag
Val 903	-	expression tag
Leu 904	-	expression tag
Phe 905	-	expression tag
Gln 906	-	expression tag
Gly 907	-	expression tag
Pro 908	-	expression tag
Ser 909	-	expression tag
Ser 910	-	expression tag
Gly 911	-	expression tag
His 912	-	expression tag
His 913	-	expression tag
His 914	-	expression tag
His 915	-	expression tag
His 916	-	expression tag
His 917	-	expression tag
His 918	-	expression tag
His 919	-	expression tag
His 920	-	expression tag
His 921	-	expression tag

C, D: 896 - 1010 (UniProt: Q0P7J9)

PDB	External Database	Details
Lys 1011	-	expression tag
Leu 1012	-	expression tag
Ser 1013	-	expression tag
Ser 1014	-	expression tag
Gly 1015	-	expression tag
Leu 1016	-	expression tag
Glu 1017	-	expression tag
Val 1018	-	expression tag
Leu 1019	-	expression tag
Phe 1020	-	expression tag
Gln 1021	-	expression tag
Gly 1022	-	expression tag
Pro 1023	-	expression tag
Ser 1024	-	expression tag
Ser 1025	-	expression tag
Gly 1026	-	expression tag
His 1027	-	expression tag
His 1028	-	expression tag
His 1029	-	expression tag
His 1030	-	expression tag
His 1031	-	expression tag
His 1032	-	expression tag
His 1033	-	expression tag
His 1034	-	expression tag
His 1035	-	expression tag
His 1036	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	228989.9
All*	Total formula weight	228989.9

*Water molecules are not included.