8XJM

Latanoprost acid bound Prostaglandin F2-alpha receptor-Gq Protein Complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Engineered miniGq	polymer	246	28084.8	1		synthetic construct
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	376	40845.6	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
3	C	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
4	E	Antibody fragment scFv16	polymer	254	27259.3	1		synthetic construct
5	R	Fusion tag,Prostaglandin F2-alpha receptor,LgBiT	polymer	588	64767.3	1	UniProt (P43088) Pfam (PF00001) In PDB	synthetic construct	PGF receptor,PGF2-alpha receptor,Prostanoid FP receptor
6	R	Z-7-[(1R,2R,3R,5S)-3,5-bis(oxidanyl)-2-[(3R)-3-oxidanyl-5-phenyl-pentyl]cyclopentyl]hept-5-enoic acid	non-polymer		390.5	1	Chemie (7WT)

Sequence modifications

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -9	-	initiating methionine
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag
Gly 341	-	expression tag
Ser 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Gly 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Ser 354	-	expression tag
Gly 355	-	expression tag
Val 356	-	expression tag
Ser 357	-	expression tag
Gly 358	-	expression tag
Trp 359	-	expression tag
Arg 360	-	expression tag
Leu 361	-	expression tag
Phe 362	-	expression tag
Lys 363	-	expression tag
Lys 364	-	expression tag
Ile 365	-	expression tag
Ser 366	-	expression tag

R: -84 - 0 (PDB: 8XJM)
R: 1 - 327 (UniProt: P43088)
R: 328 - 503 (PDB: 8XJM)

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	168818.1
Non-Polymers*	Number of molecules	1
	Total formula weight	390.5
All*	Total formula weight	169208.7

*Water molecules are not included.