8XIZ

Crystal structure of an epoxide hydrolase mutant A250IC/L344V from Aspergillus usamii E001 at 2.17 Angstroms resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Microsomal epoxide hyddrolase	polymer	431	48524.7	2	UniProt (T2B4K5) Pfam (PF06441)	Aspergillus usamii
2	C, D (A, B)	water	water		18.0	465	Chemie (HOH)

Sequence modifications

A, B: 1 - 395 (UniProt: T2B4K5)

PDB	External Database	Details
Met -35	-	initiating methionine
Gly -34	-	expression tag
Ser -33	-	expression tag
Ser -32	-	expression tag
His -31	-	expression tag
His -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
Ser -25	-	expression tag
Ser -24	-	expression tag
Gly -23	-	expression tag
Leu -22	-	expression tag
Val -21	-	expression tag
Pro -20	-	expression tag
Arg -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
Met -15	-	expression tag
Ala -14	-	expression tag
Ser -13	-	expression tag
Met -12	-	expression tag
Thr -11	-	expression tag
Gly -10	-	expression tag
Gly -9	-	expression tag
Gln -8	-	expression tag
Gln -7	-	expression tag
Met -6	-	expression tag
Gly -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Glu -1	-	expression tag
Phe 0	-	expression tag
Ile 250	Ala 250	engineered mutation
Val 344	Leu 344	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	97049.4
All*	Total formula weight	97049.4

*Water molecules are not included.