8XIY

Crystal structure of an omega-transaminase mutant I77L/Q97E/H210N/N245D from Aspergillus terreus in complex with PLP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	D-aminoacid aminotransferase-like PLP-dependent enzyme	polymer	335	37377.7	2	UniProt (Q0C8G1) Pfam (PF01063)	Aspergillus terreus NIH2624
2	C, D (A, B)	PYRIDOXAL-5'-PHOSPHATE	non-polymer		247.1	2	Chemie (PLP)
3	E, F (A, B)	water	water		18.0	497	Chemie (HOH)

Sequence modifications

A, B: 1 - 325 (UniProt: Q0C8G1)

PDB	External Database	Details
Met -9	-	initiating methionine
Gly -8	-	expression tag
Ser -7	-	expression tag
Ser -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Leu 77	Ile 77	engineered mutation
Glu 97	Gln 97	engineered mutation
Asn 210	His 210	engineered mutation
Asp 245	Asn 245	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	74755.3
Non-Polymers*	Number of molecules	2
	Total formula weight	494.3
All*	Total formula weight	75249.6

*Water molecules are not included.