8XIL
Cellodextrin phosphorylase from Clostridium thermocellum mutant - all cysteine residues were substituted with serines
Replaces: 8H2WEntity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Cellodextrin phosphorylase | polymer | 992 | 112679.1 | 2 | UniProt (Q93HT8) Pfam (PF06165) Pfam (PF17167) | Acetivibrio thermocellus | |
| 2 | C, D (C, D) | beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose | branched | 504.4 | 2 | In PDB BIRD (PRD_900021) GlyTouCan (G14338VK) | |||
| 3 | CA, DA, E, F, FA... (B, A) | ACETATE ION | non-polymer | 59.0 | 8 | Chemie (ACT) | |||
| 4 | BA, EA, G (B, A) | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 3 | Chemie (TRS) | |||
| 5 | GA, K (B, A) | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
| 6 | HA, IA, L, M, N (B, A) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 5 | Chemie (PEG) | |||
| 7 | JA, KA, LA, MA, NA... (B, A) | GLYCEROL | non-polymer | 92.1 | 23 | Chemie (GOL) | |||
| 8 | AA, VA, WA, XA, Z (A, B) | CHLORIDE ION | non-polymer | 35.5 | 5 | Chemie (CL) | |||
| 9 | YA, ZA (A, B) | water | water | 18.0 | 2099 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 984 (UniProt: Q93HT8)
| PDB | External Database | Details |
|---|---|---|
| Ser 64 | Cys 64 | engineered mutation |
| Ser 80 | Cys 80 | engineered mutation |
| Ser 225 | Cys 225 | engineered mutation |
| Ser 229 | Cys 229 | engineered mutation |
| Ser 240 | Cys 240 | engineered mutation |
| Ser 353 | Cys 353 | engineered mutation |
| Ser 372 | Cys 372 | engineered mutation |
| Ser 606 | Cys 606 | engineered mutation |
| Ser 625 | Cys 625 | engineered mutation |
| Asp 629 | Ala 629 | engineered mutation |
| Ser 872 | Cys 872 | engineered mutation |
| Ser 934 | Cys 934 | engineered mutation |
| Leu 985 | - | expression tag |
| Glu 986 | - | expression tag |
| His 987 | - | expression tag |
| His 988 | - | expression tag |
| His 989 | - | expression tag |
| His 990 | - | expression tag |
| His 991 | - | expression tag |
| His 992 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 225358.3 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 1008.9 | |
| Non-Polymers* | Number of molecules | 46 |
| Total formula weight | 3856.9 | |
| All* | Total formula weight | 230224.1 |
