8XBW

The cryo-EM structure of the RAD51 N-terminal lobe domain bound to the histone H4 tail of the nucleosome

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (E)	Histone H3.1	polymer	139	15719.4	1	UniProt (P68431) Pfam (PF00125)	Homo sapiens (human)	Histone H3/a,Histone H3/b,Histone H3/c,Histone H3/d,Histone H3/f,Histone H3/h,Histone H3/i,Histone H3/j,Histone H3/k,Histone H3/l
2	B (F)	Histone H4	polymer	106	11676.7	1	UniProt (P62805) Pfam (PF15511)	Homo sapiens (human)
3	C (I)	DNA (5'-D(P*AP*CP*CP*GP*CP*TP*TP*AP*AP*AP*CP*GP*CP*AP*CP*GP*TP*A)-3')	polymer	156	47976.7	1		synthetic construct
4	D (J)	DNA (5'-D(P*TP*AP*CP*GP*TP*GP*CP*GP*TP*TP*TP*AP*AP*GP*CP*GP*GP*T)-3')	polymer	153	47371.1	1		synthetic construct
5	E (L)	DNA repair protein RAD51 homolog 1	polymer	342	37291.4	1	UniProt (Q06609) Pfam (PF14520) Pfam (PF08423)	Homo sapiens (human)	HsRAD51,hRAD51,RAD51 homolog A

Sequence modifications

E: 0 - 135 (UniProt: P68431)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag

F: 0 - 102 (UniProt: P62805)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag

L: 1 - 339 (UniProt: Q06609)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	160035.3
All*	Total formula weight	160035.3

*Water molecules are not included.