8XA7
Cryo-EM structure of Bacillus subtilis RNAP,sigA and SPO1 gp33 complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | DNA-directed RNA polymerase subunit alpha | polymer | 314 | 34842.4 | 2 | UniProt (P20429) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) | Bacillus | |
| 2 | C (C) | DNA-directed RNA polymerase subunit beta | polymer | 1193 | 133847.9 | 1 | UniProt (P37870) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) | Bacillus | |
| 3 | D (D) | DNA-directed RNA polymerase subunit beta' | polymer | 1199 | 134445.0 | 1 | UniProt (P37871) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Bacillus | |
| 4 | E (F) | DNA-directed RNA polymerase subunit omega | polymer | 67 | 7766.9 | 1 | UniProt (O35011) Pfam (PF01192) | Bacillus | |
| 5 | F (G) | RNA polymerase sigma factor SigA | polymer | 371 | 43007.4 | 1 | UniProt (P06224) Pfam (PF03979) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) | Bacillus | |
| 6 | G (H) | Gene 33 protein | polymer | 101 | 11920.5 | 1 | UniProt (P06226) | Bacillus phage SPO1 | |
| 7 | H (L) | DNA-directed RNA polymerase subunit delta | polymer | 173 | 20417.9 | 1 | UniProt (P12464) Pfam (PF05066) | Bacillus | |
| 8 | I (E) | DNA-directed RNA polymerase subunit epsilon | polymer | 69 | 8277.4 | 1 | UniProt (O31718) Pfam (PF07288) | Bacillus |
Sequence modifications
E: 1 - 69 (UniProt: O31718)
| PDB | External Database | Details |
|---|---|---|
| Ile 33 | Val 33 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 429367.8 | |
| All* | Total formula weight | 429367.8 |
