8X8L

Cryo-EM structure of the cortistatin 17-bound Somatostatin receptor 5-Gi protein complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(i) subunit alpha	polymer	360	41510.0	1		Homo sapiens
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	371	40227.0	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
3	C (C)	Cortistatin	polymer	17	2157.5	1	UniProt (O00230) Pfam (PF03002)	Homo sapiens
4	D (E)	ScFv16	polymer	248	26480.5	1		Mus musculus
5	E (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	70	7729.9	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
6	F (R)	Somatostatin receptor type 5	polymer	364	39249.2	1	UniProt (P35346) Pfam (PF00001)	Homo sapiens (human)

Sequence modifications

B: 7 - 345 (UniProt: P62873)

PDB	External Database	Details
Met 1	-	initiating methionine
Gly 2	-	expression tag
Ser 3	-	expression tag
Leu 4	-	expression tag
Leu 5	-	expression tag
Gln 6	-	expression tag
Gly 346	-	expression tag
Ser 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Gly 354	-	expression tag
Gly 355	-	expression tag
Gly 356	-	expression tag
Gly 357	-	expression tag
Ser 358	-	expression tag
Ser 359	-	expression tag
Gly 360	-	expression tag
Val 361	-	expression tag
Ser 362	-	expression tag
Gly 363	-	expression tag
Trp 364	-	expression tag
Arg 365	-	expression tag
Leu 366	-	expression tag
Phe 367	-	expression tag
Lys 368	-	expression tag
Lys 369	-	expression tag
Ile 370	-	expression tag
Ser 371	-	expression tag

R: 1 - 364 (UniProt: P35346)

PDB	External Database	Details
Leu 253	Val 253	conflict

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	157354.2
All*	Total formula weight	157354.2

*Water molecules are not included.