8X4P
Crystal structure of the Y135A mutant of DIMT1 in complex with adenosylornithine (SFG) from Pyrococcus horikoshii
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Probable ribosomal RNA small subunit methyltransferase A | polymer | 290 | 33273.0 | 2 | UniProt (O59487) Pfam (PF00398) | Pyrococcus horikoshii OT3 | |
| 2 | C, D, E, F (A) | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) | |||
| 3 | G, H (A, B) | SINEFUNGIN | non-polymer | 381.4 | 2 | Chemie (SFG) | |||
| 4 | I, J (A, B) | water | water | 18.0 | 102 | Chemie (HOH) |
Sequence modifications
A, B: 23 - 290 (UniProt: O59487)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Ser 2 | - | expression tag |
| Ser 3 | - | expression tag |
| Arg 4 | - | expression tag |
| Ile 5 | - | expression tag |
| Arg 6 | - | expression tag |
| Ile 7 | - | expression tag |
| Asn 8 | - | expression tag |
| Thr 9 | - | expression tag |
| Lys 10 | - | expression tag |
| Ser 11 | - | expression tag |
| Leu 12 | - | expression tag |
| Leu 13 | - | expression tag |
| Val 14 | - | expression tag |
| Gln 15 | - | expression tag |
| Pro 16 | - | expression tag |
| Gly 17 | - | expression tag |
| Tyr 18 | - | expression tag |
| Ser 19 | - | expression tag |
| Gly 20 | - | expression tag |
| Ser 21 | - | expression tag |
| Lys 22 | - | expression tag |
| Ala 135 | Tyr 113 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 66546.0 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 1024.4 | |
| All* | Total formula weight | 67570.5 |
