8X4O
Crystal structure of the Y135A mutant of DIMT1 in complex with 5'-methylthioadenosine from Pyrococcus horikoshii
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Probable ribosomal RNA small subunit methyltransferase A | polymer | 290 | 33273.0 | 2 | UniProt (O59487) | Pyrococcus horikoshii OT3 | |
| 2 | C, F (A, B) | 5'-DEOXY-5'-METHYLTHIOADENOSINE | non-polymer | 297.3 | 2 | Chemie (MTA) PubChem (439176) | |||
| 3 | D, G (A, B) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) PubChem (32051) | |||
| 4 | E, H (A, B) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 2 | Chemie (EDO) | |||
| 5 | I, J (A, B) | water | water | 18.0 | 21 | Chemie (HOH) |
Sequence modifications
A, B: 23 - 290 (UniProt: O59487)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Ser 2 | - | expression tag |
| Ser 3 | - | expression tag |
| Arg 4 | - | expression tag |
| Ile 5 | - | expression tag |
| Arg 6 | - | expression tag |
| Ile 7 | - | expression tag |
| Asn 8 | - | expression tag |
| Thr 9 | - | expression tag |
| Lys 10 | - | expression tag |
| Ser 11 | - | expression tag |
| Leu 12 | - | expression tag |
| Leu 13 | - | expression tag |
| Val 14 | - | expression tag |
| Gln 15 | - | expression tag |
| Pro 16 | - | expression tag |
| Gly 17 | - | expression tag |
| Tyr 18 | - | expression tag |
| Ser 19 | - | expression tag |
| Gly 20 | - | expression tag |
| Ser 21 | - | expression tag |
| Lys 22 | - | expression tag |
| Ala 135 | Tyr 113 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 66546.0 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 849.6 | |
| All* | Total formula weight | 67395.7 |
