8X4O

Crystal structure of the Y135A mutant of DIMT1 in complex with 5'-methylthioadenosine from Pyrococcus horikoshii

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Probable ribosomal RNA small subunit methyltransferase A	polymer	290	33273.0	2	UniProt (O59487) In PDB	Pyrococcus horikoshii OT3
2	A, B	5'-DEOXY-5'-METHYLTHIOADENOSINE	non-polymer		297.3	2	Chemie (MTA)
3	A, B	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4	A, B	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
5		water	water		18.0	21	Chemie (HOH)

Sequence modifications

A, B: 23 - 290 (UniProt: O59487)

PDB	External Database	Details
Met 1	-	initiating methionine
Ser 2	-	expression tag
Ser 3	-	expression tag
Arg 4	-	expression tag
Ile 5	-	expression tag
Arg 6	-	expression tag
Ile 7	-	expression tag
Asn 8	-	expression tag
Thr 9	-	expression tag
Lys 10	-	expression tag
Ser 11	-	expression tag
Leu 12	-	expression tag
Leu 13	-	expression tag
Val 14	-	expression tag
Gln 15	-	expression tag
Pro 16	-	expression tag
Gly 17	-	expression tag
Tyr 18	-	expression tag
Ser 19	-	expression tag
Gly 20	-	expression tag
Ser 21	-	expression tag
Lys 22	-	expression tag
Ala 135	Tyr 113	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	66546.0
Non-Polymers*	Number of molecules	6
	Total formula weight	849.6
All*	Total formula weight	67395.7

*Water molecules are not included.