8X4I

Crystal structure of the D117A mutant of DIMT1 in complex with 5'-methylthioadenosine from Pyrococcus horikoshii

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Probable ribosomal RNA small subunit methyltransferase A	polymer	290	33321.1	1	UniProt (O59487) Pfam (PF00398) In PDB	Pyrococcus horikoshii OT3
2	A	5'-DEOXY-5'-METHYLTHIOADENOSINE	non-polymer		297.3	1	Chemie (MTA)
3	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
4		water	water		18.0	6	Chemie (HOH)

Sequence modifications

A: 23 - 290 (UniProt: O59487)

PDB	External Database	Details
Met 1	-	initiating methionine
Ser 2	-	expression tag
Ser 3	-	expression tag
Arg 4	-	expression tag
Ile 5	-	expression tag
Arg 6	-	expression tag
Ile 7	-	expression tag
Asn 8	-	expression tag
Thr 9	-	expression tag
Lys 10	-	expression tag
Ser 11	-	expression tag
Leu 12	-	expression tag
Leu 13	-	expression tag
Val 14	-	expression tag
Gln 15	-	expression tag
Pro 16	-	expression tag
Gly 17	-	expression tag
Tyr 18	-	expression tag
Ser 19	-	expression tag
Gly 20	-	expression tag
Ser 21	-	expression tag
Lys 22	-	expression tag
Ala 117	Asp 95	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	33321.1
Non-Polymers*	Number of molecules	2
	Total formula weight	362.7
All*	Total formula weight	33683.9

*Water molecules are not included.