8X17

Cryo-EM structure of adenosine receptor A3AR bound to CF102

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	345	37915.5	1	UniProt (P54311) Pfam (PF00400) In PDB	Rattus norvegicus (Norway rat)	Transducin beta chain 1
2	R	Adenosine receptor A3	polymer	318	36214.5	1	UniProt (P0DMS8) Pfam (PF00001) In PDB	Homo sapiens (human)
3	C	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P63212) Pfam (PF00631) In PDB	Bos taurus (cattle)	G gamma-I
4	H	scFv16	polymer	247	26277.3	1		Mus musculus
5	A	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40445.1	1	UniProt (P63097) Pfam (PF00503) In PDB	Bos taurus (cattle)
6	R	namodenoson	non-polymer		544.7	1	Chemie (XS0)

Sequence modifications

B: 2 - 340 (UniProt: P54311)

PDB	External Database	Details
Met -4	-	initiating methionine
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

A: 1 - 354 (UniProt: P63097)

PDB	External Database	Details
Ala 203	Gly 203	engineered mutation
Ser 326	Ala 326	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	148713.5
Non-Polymers*	Number of molecules	1
	Total formula weight	544.7
All*	Total formula weight	149258.2

*Water molecules are not included.