8WYF

Cryo-EM structure of DSR2-DSAD1-NAD+ (partial) complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	SIR2 family protein	polymer	1005	118635.8	4		Bacillus subtilis
2	E (E)	Bacillus phage SPbeta DSAD1 protein	polymer	146	16587.8	1	UniProt (O64191)	Bacillus phage SPBc2
3	F, G, H, I (A, B, C, D)	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	4	Chemie (NAD)

Sequence modifications

E: 1 - 120 (UniProt: O64191)

PDB	External Database	Details
Trp 121	-	expression tag
Ser 122	-	expression tag
His 123	-	expression tag
Pro 124	-	expression tag
Gln 125	-	expression tag
Phe 126	-	expression tag
Glu 127	-	expression tag
Lys 128	-	expression tag
Gly 129	-	expression tag
Gly 130	-	expression tag
Gly 131	-	expression tag
Ser 132	-	expression tag
Gly 133	-	expression tag
Gly 134	-	expression tag
Gly 135	-	expression tag
Ser 136	-	expression tag
Gly 137	-	expression tag
Gly 138	-	expression tag
Trp 139	-	expression tag
Ser 140	-	expression tag
His 141	-	expression tag
Pro 142	-	expression tag
Gln 143	-	expression tag
Phe 144	-	expression tag
Glu 145	-	expression tag
Lys 146	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	491130.9
Non-Polymers*	Number of molecules	4
	Total formula weight	2653.7
All*	Total formula weight	493784.6

*Water molecules are not included.