8WUS

SpCas9-MMLV RT-pegRNA-target DNA complex (termination)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	E	Gag-Pol polyprotein	polymer	496	55656.2	1	UniProt (Q8UN00) Pfam (PF00078) Pfam (PF17919) In PDB	Moloney murine leukemia virus
2	A	CRISPR-associated endonuclease Cas9/Csn1	polymer	1367	158457.6	1	UniProt (Q99ZW2) Pfam (PF16593) Pfam (PF16592) Pfam (PF13395) Pfam (PF22702) Pfam (PF16595) In PDB	Streptococcus pyogenes serotype M1
3	C	DNA (51-MER)	polymer	51	15636.0	1		Streptococcus pyogenes
4	D	DNA (5'-D(*TP*GP*AP*TP*GP*GP*CP*AP*GP*AP*GP*TP*AP*CP*TP*AP*G)-3')	polymer	17	5291.4	1		Streptococcus pyogenes
5	B	RNA (115-MER)	polymer	115	37065.9	1		Streptococcus pyogenes
6	F	DNA (40-MER)	polymer	40	12315.9	1		Streptococcus pyogenes

Sequence modifications

E: 1 - 496 (UniProt: Q8UN00)

PDB	External Database	Details
Asn 200	Asp 859	engineered mutation
Asn 249	Asp 908	engineered mutation
Lys 306	Thr 965	engineered mutation
Phe 313	Trp 972	engineered mutation
Pro 330	Thr 989	engineered mutation
Asn 335	Asp 994	engineered mutation

A: 2 - 1368 (UniProt: Q99ZW2)

PDB	External Database	Details
Ala 10	Asp 10	engineered mutation
Ala 840	His 840	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	284423.2
All*	Total formula weight	284423.2

*Water molecules are not included.