8WT7
Cryo-EM structure of the IS621 recombinase in complex with bridge RNA, donor DNA, and target DNA in the pre-strand exchange locked state
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | IS621 transposase | polymer | 328 | 36735.4 | 4 | UniProt (A0A0E0Y1P1) Pfam (PF01548) Pfam (PF02371) UniProt (by SIFTS) (A0A2X3M8B0) | Escherichia coli | |
| 2 | E, F (E, F) | bridge RNA | polymer | 180 | 57668.9 | 2 | Escherichia coli | ||
| 3 | G (G) | target DNA | polymer | 38 | 11659.5 | 1 | Escherichia coli | ||
| 4 | H (H) | target DNA | polymer | 38 | 11703.5 | 1 | Escherichia coli | ||
| 5 | I (I) | donor DNA | polymer | 44 | 13507.7 | 1 | Escherichia coli | ||
| 6 | J (J) | donor DNA | polymer | 44 | 13587.7 | 1 | Escherichia coli | ||
| 7 | K, L (A, C) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 8 | M, N (A, C) | water | water | 18.0 | 4 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 326 (UniProt: A0A0E0Y1P1)
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| Pro 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 312737.7 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 48.6 | |
| All* | Total formula weight | 312786.3 |
