8WQF

cryo-EM structure of CUL2-RBX1-ELOB-ELOC-FEM1B bound with the C-degron of CUX1 (conformation 2)

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Cullin-2	polymer	750	87114.8	2	UniProt (Q13617) Pfam (PF00888) Pfam (PF10557) In PDB	Homo sapiens (human)	CUL-2
2	G, C	Elongin-C	polymer	96	10843.4	2	UniProt (Q15369) Pfam (PF03931) In PDB	Homo sapiens (human)	EloC,Elongin 15 kDa subunit,RNA polymerase II transcription factor SIII subunit C,SIII p15,Transcription elongation factor B polypeptide 1
3	H, D	Elongin-B	polymer	118	13147.8	2	UniProt (Q15370) Pfam (PF00240) In PDB	Homo sapiens (human)	EloB,Elongin 18 kDa subunit,RNA polymerase II transcription factor SIII subunit B,SIII p18,Transcription elongation factor B polypeptide 2
4	I, E	E3 ubiquitin-protein ligase RBX1, N-terminally processed	polymer	96	11196.8	2	UniProt (P62877) Pfam (PF12678) In PDB	Homo sapiens (human)	E3 ubiquitin-protein transferase RBX1,N-terminally processed
5	F, J	Protein fem-1 homolog B	polymer	627	70355.1	2	UniProt (Q9UK73) Pfam (PF12796) In PDB	Homo sapiens (human)	FEM1b,FEM1-beta,Fem-1-like death receptor-binding protein alpha,Fem-1-like in apoptotic pathway protein alpha,F1A-alpha
6	K	Protein CASP	polymer	31	3143.3	1	UniProt (Q13948) In PDB	Homo sapiens (human)
7	I, E	ZINC ION	non-polymer		65.4	6	Chemie (ZN)

Sequence modifications

A, B: 2 - 745 (UniProt: Q13617)

PDB	External Database	Details
Ser -19	-	expression tag
Ala -18	-	expression tag
Ser -17	-	expression tag
Trp -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
Pro -13	-	expression tag
Gln -12	-	expression tag
Phe -11	-	expression tag
Glu -10	-	expression tag
Lys -9	-	expression tag
Gly -8	-	expression tag
Gly -7	-	expression tag
Gly -6	-	expression tag
Ser -5	-	expression tag
Gly -4	-	expression tag
Gly -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Thr 1	-	expression tag
-	Lys 117	deletion
-	Leu 118	deletion
-	Thr 119	deletion
-	Glu 120	deletion
-	Ala 121	deletion
-	Asp 122	deletion
-	Leu 123	deletion
-	Gln 124	deletion
-	Tyr 125	deletion
-	Gly 126	deletion
-	Tyr 127	deletion
-	Gly 128	deletion
-	Gly 129	deletion
-	Val 130	deletion
-	Asp 131	deletion
Gly 132	Met 132	conflict
Gly 133	Asn 133	conflict
Gly 134	Glu 134	conflict

I, E: 16 - 108 (UniProt: P62877)

PDB	External Database	Details
Ser 13	-	expression tag
His 14	-	expression tag
Met 15	-	expression tag

K: -23 - -1 (UniProt: Q13948)

PDB	External Database	Details
Gly -31	-	expression tag
Gly -30	-	expression tag
Gly -29	-	expression tag
Ser -28	-	expression tag
Gly -27	-	expression tag
Gly -26	-	expression tag
Gly -25	-	expression tag
Ser -24	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	388459.0
Non-Polymers*	Number of molecules	6
	Total formula weight	392.5
All*	Total formula weight	388851.4

*Water molecules are not included.