8WM8

Cryo-EM structure of cyanobacterial nitrate/nitrite transporter NrtBCD in complex with nitrate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	D	Nitrate transport ATP-binding protein	polymer	277	31399.1	1	UniProt (Q8YZ75) Pfam (PF00005) In PDB	Nostoc sp.
2	B, A	Nitrate transport permease protein	polymer	279	30483.1	2	UniProt (Q8YZ77) Pfam (PF00528) In PDB	Nostoc sp.	ABC nitrate transport permease protein
3	C	Nitrate transport ATP-binding protein	polymer	682	76326.1	1	UniProt (Q8YZ76) Pfam (PF00005) Pfam (PF13379) In PDB	Nostoc sp.
4	A	NITRATE ION	non-polymer		62.0	1	Chemie (NO3)

Sequence modifications

C: 1 - 657 (UniProt: Q8YZ76)

PDB	External Database	Details
Ser 658	-	expression tag
Ser 659	-	expression tag
Gly 660	-	expression tag
Asp 661	-	expression tag
Tyr 662	-	expression tag
Lys 663	-	expression tag
Asp 664	-	expression tag
His 665	-	expression tag
Asp 666	-	expression tag
Gly 667	-	expression tag
Asp 668	-	expression tag
Tyr 669	-	expression tag
Lys 670	-	expression tag
Asp 671	-	expression tag
His 672	-	expression tag
Asp 673	-	expression tag
Ile 674	-	expression tag
Asp 675	-	expression tag
Tyr 676	-	expression tag
Lys 677	-	expression tag
Asp 678	-	expression tag
Asp 679	-	expression tag
Asp 680	-	expression tag
Asp 681	-	expression tag
Lys 682	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	168691.4
Non-Polymers*	Number of molecules	1
	Total formula weight	62.0
All*	Total formula weight	168753.4

*Water molecules are not included.