8WM7

Cryo-EM structure of cyanobacterial nitrate/nitrite transporter NrtBCD in complex with signalling protein PII

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (B, A)	Nitrate transport permease protein	polymer	279	30483.1	2	UniProt (Q8YZ77) Pfam (PF00528)	Nostoc sp.	ABC nitrate transport permease protein
2	C (C)	Nitrate transport ATP-binding protein	polymer	682	76326.1	1	UniProt (Q8YZ76) Pfam (PF00005) Pfam (PF13379)	Nostoc sp.
3	D (D)	Nitrate transport ATP-binding protein	polymer	277	31399.1	1	UniProt (Q8YZ75) Pfam (PF00005)	Nostoc sp.
4	E, F, G (E, F, G)	Nitrogen regulatory protein P-II	polymer	118	13339.4	3	UniProt (Q9L422) Pfam (PF00543)	Nostoc sp.
5	H, I, J, K, L (C, D, E, F)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	5	Chemie (ADP)

Sequence modifications

C: 1 - 657 (UniProt: Q8YZ76)

PDB	External Database	Details
Ser 658	-	expression tag
Ser 659	-	expression tag
Gly 660	-	expression tag
Asp 661	-	expression tag
Tyr 662	-	expression tag
Lys 663	-	expression tag
Asp 664	-	expression tag
His 665	-	expression tag
Asp 666	-	expression tag
Gly 667	-	expression tag
Asp 668	-	expression tag
Tyr 669	-	expression tag
Lys 670	-	expression tag
Asp 671	-	expression tag
His 672	-	expression tag
Asp 673	-	expression tag
Ile 674	-	expression tag
Asp 675	-	expression tag
Tyr 676	-	expression tag
Lys 677	-	expression tag
Asp 678	-	expression tag
Asp 679	-	expression tag
Asp 680	-	expression tag
Asp 681	-	expression tag
Lys 682	-	expression tag

E, F, G: 1 - 112 (UniProt: Q9L422)

PDB	External Database	Details
His 113	-	expression tag
His 114	-	expression tag
His 115	-	expression tag
His 116	-	expression tag
His 117	-	expression tag
His 118	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	208709.6
Non-Polymers*	Number of molecules	5
	Total formula weight	2136.0
All*	Total formula weight	210845.6

*Water molecules are not included.