8WLZ

Cryo-EM structure of the WIV1 S-hACE2 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	Spike glycoprotein,Fibritin	polymer	1271	140747.7	3	UniProt (U5WI05) UniProt (A0A346FJN8) Pfam (PF16451) Pfam (PF09408) Pfam (PF01601) In PDB	Bat SARS-like coronavirus WIV1	S glycoprotein,E2,Peplomer protein,Phage neck whiskers protein
2	D, G	Processed angiotensin-converting enzyme 2	polymer	603	69982.6	2	UniProt (Q9BYF1) Pfam (PF01401) In PDB	Homo sapiens (human)
3	E, F, H, I, J...	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	13	In PDB GlyTouCan (G42666HT)
4	M, N, S	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	3	In PDB GlyTouCan (G15407YE)
5	B, C, A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	21	Chemie (NAG)

Sequence modifications

A, B, C: 1 - 1191 (UniProt: U5WI05)

PDB	External Database	Details
Pro 969	Lys 969	conflict
Pro 970	Val 970	conflict
Gly 1192	-	linker
Ser 1193	-	linker

A, B, C: 1194 - 1219 (UniProt: A0A346FJN8)

PDB	External Database	Details
Leu 1220	-	expression tag
Gly 1221	-	expression tag
Arg 1222	-	expression tag
Ser 1223	-	expression tag
Leu 1224	-	expression tag
Glu 1225	-	expression tag
Val 1226	-	expression tag
Leu 1227	-	expression tag
Phe 1228	-	expression tag
Gln 1229	-	expression tag
Gly 1230	-	expression tag
Pro 1231	-	expression tag
Gly 1232	-	expression tag
His 1233	-	expression tag
His 1234	-	expression tag
His 1235	-	expression tag
His 1236	-	expression tag
His 1237	-	expression tag
His 1238	-	expression tag
His 1239	-	expression tag
His 1240	-	expression tag
Ser 1241	-	expression tag
Ala 1242	-	expression tag
Trp 1243	-	expression tag
Ser 1244	-	expression tag
His 1245	-	expression tag
Pro 1246	-	expression tag
Gln 1247	-	expression tag
Phe 1248	-	expression tag
Glu 1249	-	expression tag
Lys 1250	-	expression tag
Gly 1251	-	expression tag
Gly 1252	-	expression tag
Gly 1253	-	expression tag
Ser 1254	-	expression tag
Gly 1255	-	expression tag
Gly 1256	-	expression tag
Gly 1257	-	expression tag
Gly 1258	-	expression tag
Ser 1259	-	expression tag
Gly 1260	-	expression tag
Gly 1261	-	expression tag
Ser 1262	-	expression tag
Ala 1263	-	expression tag
Trp 1264	-	expression tag
Ser 1265	-	expression tag
His 1266	-	expression tag
Pro 1267	-	expression tag
Gln 1268	-	expression tag
Phe 1269	-	expression tag
Glu 1270	-	expression tag
Lys 1271	-	expression tag

D, G: 19 - 615 (UniProt: Q9BYF1)

PDB	External Database	Details
His 616	-	expression tag
His 617	-	expression tag
His 618	-	expression tag
His 619	-	expression tag
His 620	-	expression tag
His 621	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	562208.2
Branched	Number of molecules	16
	Total formula weight	7276.8
Non-Polymers*	Number of molecules	21
	Total formula weight	4645.4
All*	Total formula weight	574130.4

*Water molecules are not included.